Signal on fluorescence biosensor for MMP-2 based on FRET between semiconducting polymer dots and a metal organic framework (original) (raw)
* Corresponding authors
a MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, China
E-mail: zylin @fzu.edu.cn
b Department of Chemistry and Environmental Science, Minnan Normal University, Zhangzhou, China
E-mail: weng_wen@126.com
c Food and Biological Engineering, Zhangzhou Institute of Technology, China
Abstract
A signal on fluorescence biosensor for ultrasensitive detection of MMP-2 based on fluorescence resonance energy transfer (FRET) between semiconducting polymer dots (Pdots) and a metal–organic framework (MOF) has been developed. Carboxyl-rich PFO has been modified firmly with a polypeptide chain (COOH–GHHYYGPLGVRGC–NH2) through a carboxy ammonia reaction first. The polypeptide chain comprises the specific MMP-2 substrate domain (PLGVR) and the π-rich motif (HHYY) can be absorbed by the MOF (H2_dtoa_Cu used in this study) through π-staking interactions. Hence, the fluorescence from Pdots can be quenched by the MOF with the assistance of the polypeptide chain linker through the FRET process. Upon the cleavage of the substrate by the protease (MMP-2) at the amide bond between Gly and Val, the Pdots has been separated from the MOF surface and therefore the FRET process can be inhibited, and the fluorescence of the system recovered. Under optimal conditions, the fluorescence recovery was found to be proportional to the concentration of MMP-2 within the range of 0.1 to 2.5 pg mL−1.
You have access to this article
Please wait while we load your content... Something went wrong. Try again?
Article information
DOI
https://doi.org/10.1039/C4RA12478B
Article type
Paper
Submitted
16 Oct 2014
Accepted
30 Oct 2014
First published
30 Oct 2014
Download Citation
RSC Adv., 2014,4, 58852-58857
Permissions
Signal on fluorescence biosensor for MMP-2 based on FRET between semiconducting polymer dots and a metal organic framework
W. Yang, G. Zhang, W. Weng, B. Qiu, L. Guo, Z. Lin and G. Chen,RSC Adv., 2014, 4, 58852DOI: 10.1039/C4RA12478B
To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.
If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.
If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.
Read more about how to correctly acknowledge RSC content.