A turn-on upconversion fluorescence resonance energy transfer biosensor for ultrasensitive endonuclease detection (original) (raw)
* Corresponding authors
a State Key Laboratory for Chemo/biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, PR China
E-mail: xiachu@hnu.edu.cn, rqyu@hnu.edu.cn
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Tel: +86 731 88821916
Abstract
A facile one-step approach was proposed to make hydrophilic and DNA-functionalizable upconversion nanoparticles through ligand exchange at the liquid–liquid interface, and an ultrasensitive and selective biosensor for nuclease assay and its inhibitors assay based on FRET from the DNA-functionalizable UCNPs to graphene oxide was designed. A high sensitivity exhibited with a detectable minimum concentration of 1 × 10−4 units per mL S1 nuclease, which was more sensitive than the developed approaches.
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Article information
DOI
https://doi.org/10.1039/C5AY01169H
Article type
Paper
Submitted
06 May 2015
Accepted
27 Jun 2015
First published
29 Jun 2015
Download Citation
Anal. Methods, 2015,7, 7474-7479
Author version available
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A turn-on upconversion fluorescence resonance energy transfer biosensor for ultrasensitive endonuclease detection
L. Huang, X. Tian, J. Yi, R. Yu and X. Chu,Anal. Methods, 2015, 7, 7474DOI: 10.1039/C5AY01169H
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