Detection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry (original) (raw)
* Corresponding authors
a University of Exeter, School of Physics and Astronomy, Exeter EX4 4QL, UK
E-mail: f.palombo@exeter.ac.uk
b University of Exeter, Medical School, Hatherly Laboratories, Exeter EX4 4PS, UK
c University of Exeter, Centre for Biomedical Modelling and Analysis, Exeter EX2 5DW, UK
d University of Perugia, Department of Physics and Geology, Perugia I-06100, Italy
e University of Reading, School of Pharmacy, Reading Hopkins building, Reading RG6 6UB, UK
Abstract
Recent work using micro-Fourier transform infrared (μFTIR) imaging has revealed that a lipid-rich layer surrounds many plaques in post-mortem Alzheimer's brain. However, the origin of this lipid layer is not known, nor is its role in the pathogenesis of Alzheimer's disease (AD). Here, we studied the biochemistry of plaques in situ using a model of AD. We combined FTIR, Raman and immunofluorescence images, showing that astrocyte processes co-localise with the lipid ring surrounding many plaques. We used μFTIR imaging to rapidly measure chemical signatures of plaques over large fields of view, and selected plaques for higher resolution analysis with Raman microscopy. Raman maps showed similar lipid rings and dense protein cores as in FTIR images, but also revealed cell bodies. We confirmed the presence of plaques using amylo-glo staining, and detected astrocytes using immunohistochemistry, revealing astrocyte co-localisation with lipid rings. This work is important because it correlates biochemical changes surrounding the plaque with the biological process of astrogliosis.
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Article information
DOI
https://doi.org/10.1039/C7AN01747B
Article type
Paper
Submitted
23 Oct 2017
Accepted
01 Dec 2017
First published
04 Dec 2017
This article is Open Access
Download Citation
Analyst, 2018,143, 850-857
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Detection of Aβ plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry
F. Palombo, F. Tamagnini, J. C. G. Jeynes, S. Mattana, I. Swift, J. Nallala, J. Hancock, J. T. Brown, A. D. Randall and N. Stone,Analyst, 2018, 143, 850DOI: 10.1039/C7AN01747B
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