Microfluidics-enabled phenotyping, imaging, and screening of multicellular organisms (original) (raw)
* Corresponding authors
a Interdisciplinary Program in Bioengineering, Georgia Institute of Technology, 311 Ferst Dr NW, Atlanta, GA, USA
E-mail: hang.lu@gatech.edu
Tel: +1-404-894-8473
b School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Dr NW, Atlanta, GA, USA
Abstract
This paper reviews the technologies that have been invented in the last few years on high-throughput phenotyping, imaging, screening, and related techniques using microfluidics. The review focuses on the technical challenges and how microfluidics can help to solve these existing problems, specifically discussing the applications of microfluidics to multicellular model organisms. The challenges facing this field include handling multicellular organisms in an efficient manner, controlling the microenvironment and precise manipulation of the local conditions to allow the phenotyping, screening, and imaging of the small animals. Not only does microfluidics have the proper length scale for manipulating these biological entities, but automation has also been demonstrated with these systems, and more importantly the ability to deliver stimuli or alter biophysical/biochemical conditions to the biological entities with good spatial and temporal controls. In addition, integration with and interfacing to other hardware/software allows quantitative approaches. We include several successful examples of microfluidics solving these high-throughput problems. The paper also highlights other applications that can be developed in the future.
You have access to this article
Please wait while we load your content... Something went wrong. Try again?
Article information
DOI
https://doi.org/10.1039/B927258E
Article type
Critical Review
Submitted
24 Dec 2009
Accepted
10 Mar 2010
First published
09 Apr 2010
Download Citation
Lab Chip, 2010,10, 1509-1517
Permissions
Microfluidics-enabled phenotyping, imaging, and screening of multicellular organisms
M. M. Crane, K. Chung, J. Stirman and H. Lu,Lab Chip, 2010, 10, 1509DOI: 10.1039/B927258E
To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.
If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.
If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.
Read more about how to correctly acknowledge RSC content.