Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer's disease brain (original) (raw)
* Corresponding authors
a Department of Chemistry, University of Manitoba, 360 Parker Building,144 Dysart Road, Winnipeg, Manitoba, Canada
E-mail: Kathleen.Gough@ad.umanitoba.ca
Fax: +1-204-474-7608
Tel: +1-204-474-6262
b Synchrotron Radiation Center, University of Wisconsin-Madison, Stoughton, WI 53589, USA
c Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba and Division of Neurodegenerative Disorders, St. Boniface Hospital Research Centre, Winnipeg, Manitoba, Canada
E-mail: BAlbensi@sbrc.ca
Fax: +1-204-237-4092
Tel: +1-204-235-3942
d Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA
E-mail: cjhirsch@uwm.edu
Fax: +1-414.229.5589
Tel: +1-414-229 5748
Abstract
While the basis of neuronal degeneration in Alzheimer's disease (AD) continues to be debated, the amyloid cascade hypothesis remains central. Amyloid plaques are a required pathological marker for post mortem diagnosis, and Aβ peptide is regarded by most as a critical trigger at the very least. We present spectrochemical image analysis of brain tissue sections obtained with the mid-infrared beamline IRENI (InfraRed ENvironmental Imaging, Synchrotron Radiation Center, U Wisconsin-Madison), where the pixel resolution of 0.54 × 0.54 µm2 permits analysis at sub-cellular dimensions. Spectrochemical images of dense core plaque found in hippocampus and cortex sections of two transgenic mouse models of AD (TgCRND8 and 3×Tg) are compared with plaque images from a 91 year old apoE43 human AD case. Spectral analysis was done in conjunction with histochemical stains of serial sections. A lipid membrane-like spectral signature surrounded and infiltrated the dense core plaques in all cases. Remarkable compositional similarities in early stage plaques suggest similar routes to plaque formation, regardless of genetic predisposition or mammalian origin.
- This article is part of the themed collection:Optical Diagnosis
This article is Open Access
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Article information
DOI
https://doi.org/10.1039/C3AN00295K
Article type
Paper
Submitted
09 Feb 2013
Accepted
28 Mar 2013
First published
28 Mar 2013
This article is Open Access
Download Citation
Analyst, 2013,138, 3991-3997
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Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer's disease brain
C. R. Liao, M. Rak, J. Lund, M. Unger, E. Platt, B. C. Albensi, C. J. Hirschmugl and K. M. Gough,Analyst, 2013, 138, 3991DOI: 10.1039/C3AN00295K
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