Serial dilution via surface energy trap-assisted magnetic droplet manipulation (original) (raw)

* Corresponding authors

a Department of Biomedical Engineering, Johns Hopkins University, 3400 North Charles Street, Clark 122, Baltimore, Maryland, USA
E-mail: thwang@jhu.edu

b Current address: Institute of Bioengineering and Nanotechnology, Singapore 138669, Singapore

c Department of Mechanical Engineering, Sidney Kimmel Comprehensive Cancer Center, Center of Cancer Nanotechnology Excellence, Johns Hopkins University, 3400 North Charles Street, Latrobe 108, Baltimore, Maryland, USA

Abstract

This paper demonstrates a facile method of generating precise serial dilutions in the form of droplets on an open surface platform. The method relies on the use of surface energy traps (SETs), etched areas of high surface energy on a Teflon coated glass substrate, to assist in the magnetic manipulation of droplets to meter and dispense liquid of defined volumes for the preparation of serial dilutions. The volume of the dispensed liquid can be precisely controlled by the size of the SETs, facilitating generation of concentration profiles of high linearity. We have applied this approach to the generation of serial dilutions of antibiotics for anti-microbial susceptibility testing (AST).

Graphical abstract: Serial dilution via surface energy trap-assisted magnetic droplet manipulation

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Article information

DOI

https://doi.org/10.1039/C3LC50915J

Article type

Paper

Submitted

06 Aug 2013

Accepted

08 Oct 2013

First published

09 Oct 2013

Download Citation

Lab Chip, 2013,13, 4827-4831

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