Reversible protein affinity-labelling using bromomaleimide-based reagents (original) (raw)
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Abstract
Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.
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Article information
DOI
https://doi.org/10.1039/C3OB40239H
Article type
Communication
Submitted
02 Feb 2013
Accepted
26 Feb 2013
First published
26 Feb 2013
This article is Open Access
Download Citation
Org. Biomol. Chem., 2013,11, 2408-2411
Permissions
Reversible protein affinity-labelling using bromomaleimide-based reagents
R. I. Nathani, V. Chudasama, C. P. Ryan, P. R. Moody, R. E. Morgan, R. J. Fitzmaurice, M. E. B. Smith, J. R. Baker and S. Caddick,Org. Biomol. Chem., 2013, 11, 2408DOI: 10.1039/C3OB40239H
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