Genetically encoded biosensors based on engineered fluorescent proteins (original) (raw)
* Corresponding authors
a Carnegie Institution for Science, 260 Panama St., Stanford, CA 94305, USA
E-mail: wfrommer@stanford.edu
Tel: +1 650-325-1521 ext. 208
b National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, 1800 E. Paul Dirac Drive, Tallahassee, Florida 32310, USA
E-mail: davidson@magnet.fsu.edu
Tel: +1 850-644-0542
c Department of Chemistry, University of Alberta, 11227 Saskatchewan Drive, Edmonton, Alberta, Canada
E-mail: robert.e.campbell@ualberta.ca
Tel: +1 780-492-1849
Abstract
Fluorescent proteins have revolutionized cell biology by allowing researchers to non-invasively peer into the inner workings of cells and organisms. While the most common applications of fluorescent proteins are to image expression, localization, and dynamics of protein chimeras, there is a growing interest in using fluorescent proteins to create biosensors for minimally invasive imaging of concentrations of ions and small molecules, the activity of enzymes, and changes in the conformation of proteins in living cells. This tutorial review provides an overview of the progress made in the development of fluorescent protein-based biosensors to date.
- This article is part of the themed collection:Green Fluorescent Protein
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Article information
DOI
https://doi.org/10.1039/B907749A
Article type
Tutorial Review
Submitted
20 Apr 2009
First published
04 Aug 2009
Download Citation
Chem. Soc. Rev., 2009,38, 2833-2841
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Genetically encoded biosensors based on engineered fluorescent proteins
W. B. Frommer, M. W. Davidson and R. E. Campbell,Chem. Soc. Rev., 2009, 38, 2833DOI: 10.1039/B907749A
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