Immobilized silver nanoparticles enhance contact killing and show highest efficacy: elucidation of the mechanism of bactericidal action of silver (original) (raw)
* Corresponding authors
a Centre for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
b WRCBB, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
c Centre of Excellence in Nanoelectronics, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
d Centre for Environmental Science and Engineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
E-mail: mitras@iitb.ac.in
Fax: +91-22-2576-4650
Tel: +91-22-2576-7854
Abstract
Antimicrobial materials with immobilized/entrapped silver nanoparticles (AgNPs) are of considerable interest. There is significant debate on the mode of bactericidal action of AgNPs, and both contact killing and/or ion mediated killing have been proposed. In this study, AgNPs were immobilized on an amine-functionalized silica surface and their bactericidal activity was studied concurrently with the silver release profile over time. This was compared with similar studies performed using colloidal AgNPs and AgCl surfaces that released Ag ions. We conclude that contact killing is the predominant bactericidal mechanism and surface immobilized nanoparticles show greater efficacy than colloidal AgNPs, as well as a higher concentration of silver ions in solution. In addition, the AgNP immobilized substrate was used multiple times with good efficacy, indicating this immobilization protocol is effective for retaining AgNPs while maintaining their disinfection potential. The antibacterial surface was found to be extremely stable in aqueous medium and no significant leaching (∼1.15% of total silver deposited) of the AgNPs was observed. Thus, immobilization of AgNPs on a surface may promote reuse, reduce environmental risks associated with leaching of AgNPs and enhance cost effectiveness.
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Article information
DOI
https://doi.org/10.1039/C3NR00024A
Article type
Paper
Submitted
03 Jan 2013
Accepted
15 May 2013
First published
20 May 2013
This article is Open Access
Download Citation
Nanoscale, 2013,5, 7328-7340
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Immobilized silver nanoparticles enhance contact killing and show highest efficacy: elucidation of the mechanism of bactericidal action of silver
S. Agnihotri, S. Mukherji and S. Mukherji,Nanoscale, 2013, 5, 7328DOI: 10.1039/C3NR00024A
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