Rac1 is required for cell proliferation and G2/M progression (original) (raw)
Research Article| August 15 1997
*Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A.
Search for other works by this author on:
*Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A.
Search for other works by this author on:
*Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A.
Search for other works by this author on:
*Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A.
Search for other works by this author on:
*Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A.
Search for other works by this author on:
†Hematology Branch, NHLBI, NIH, Bethesda, MD 20892, U.S.A.
Search for other works by this author on:
‡Cardiology Division, Johns Hopkins Hospital, Baltimore, MD 21287, U.S.A.
Search for other works by this author on:
Pascal J. GOLDSCHMIDT-CLERMONT;
Pascal J. GOLDSCHMIDT-CLERMONT
§Heart and Lung Institute, Ohio State University, Columbus, OH 43210, U.S.A.
Search for other works by this author on:
*Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A.
1To whom correspondence should be addressed.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received: March 20 1997
Revision Received: June 30 1997
Accepted: July 02 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 326 (1): 17–20.
We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.
This content is only available as a PDF.
The Biochemical Society, London © 1997
1997
You do not currently have access to this content.
Sign in
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Email address / Username ?
Password
Could not validate captcha. Please try again.