Interleukin-10 up-regulates tumour-necrosis-factor-α-related apoptosis-inducing ligand (TRAIL) gene expression in mammary epithelial cells at the involution stage (original) (raw)
Research Article| November 08 2001
∗Animal Developmental Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea
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†Department of Pathology, School of Medicine, Wonkwang University, Iksan 570-749, Korea
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‡Department of Dermatology, College of Medicine, Catholic University, Seoul 137-040, Korea
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§Department of Surgery, National Cancer Center, Goyang 411-351, Korea
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∥Department of Biological Sciences, Ewha Womans University, Seoul 120-750, Korea
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∗Animal Developmental Biology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea
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¶Department of Biology, Dongguk University, Seoul 100-715, Korea
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Publisher: Portland Press Ltd
Received: January 15 2001
Revision Received: July 23 2001
Accepted: September 11 2001
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2001
2001
Biochem J (2001) 360 (1): 31–38.
Received:
January 15 2001
Revision Received:
July 23 2001
Accepted:
September 11 2001
Although interleukin-10 (IL-10) is known to contribute to inflammation and pathogenesis in mammalian organs, little is known about its precise role in the mammary gland. We found that IL-10 levels fluctuated during the mouse mammary cycle, showing little expression at the lactation stage and the highest expression at the involution stage. To reveal the effects of IL-10 on involution, expression profiles of apoptosis-related genes were examined in mice transgenic for IL-10 as well as in IL-10−/− mice. Mild inflammatory lesions by lymphocytes were observed in the mammary glands from four of seven transgenic lines at the lactation stage. It was striking that the expression of tumour-necrosis-factor-α-related apoptosis-inducing ligand (TRAIL) among the apoptosis-related genes was elevated approx. 7-fold in the transgenic mice, whereas others were almost unchanged. Furthermore, TRAIL was down-regulated 4-fold in the IL-10−/− mice at the involution stage. Elevated expression of TRAIL and of death receptor 4 (DR4) protein was identified at the involution stage of normal mammary glands as well as at the lactation stage of the IL-10 transgenic mice. These results indicate that the elevated expression of IL-10 at the involution stage recruits lymphocytes and induces the expression of TRAIL and DR4. These phenomena might partly contribute to apoptosis in the mammary epithelial cells for entering involution.
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The Biochemical Society, London ©2001
2001
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