Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique (original) (raw)

References

• Antunez K., D’Alessandro B., Corbella E., Ramallo G., Zunino P. (2006) Honeybee viruses in Uruguay, J. Invertebr. Pathol. 93, 67–70.
  Article PubMed Google Scholar
• Bailey L., Ball B.V. (1991) Honey Bee Pathology, London: Harcourt Brace Jovanovich.
  Google Scholar
• Bakonyi T., Farkas R., Szendroi A., Dobos-Kovacs M., Rusvai M. (2002) Detection of acute bee paralysis virus by RT-PCR in honey bee and Varroa destructor field samples: rapid screening of representative Hungarian apiaries, Apidologie 33, 63–74.
  Article CAS Google Scholar
• Benjeddou M., Leat N., Allsopp M., Davison S. (2001) Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR, Appl. Environ. Microbiol. 67, 2384–2387.
  Article PubMed CAS Google Scholar
• Berenyi O., Bakonyi T., Derakhshifar I., Koglberger H., Nowotny N. (2006) Occurrence of six honeybee viruses in diseased Austrian apiaries, Appl. Environ. Microbiol. 72, 2414–2420.
  Article PubMed CAS Google Scholar
• Blanchard P., Ribière M., Celle O., Lallemand P., Schurr F., Olivier V., Iscache A.L., Faucon J.P. (2006) Evaluation of a real-time two-step RT-PCR assay for quantitation of Chronic bee paralysis virus (CBPV) genome in experimentally-infected bee tissues and in life stages of a symptomatic colony, J. Virol. Methods 11, 11.
  Google Scholar
• Bowen-Walker P.L., Martin S.J., Gunn A. (1999) The transmission of deformed wing virus between honeybees (Apis mellifera L.) by the ectoparasitic mite Varroa jacobsoni Oud, J. Invertebr. Pathol. 73, 101–106.
  Article PubMed Google Scholar
• Bustin S.A., Nolan T. (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction, J. Biomol. Tech. 15, 155–166.
  PubMed Google Scholar
• Chantawannakul P., Ward L., Boonham N., Brown M. (2006) A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary, J. Invertebr. Pathol. 91, 69–73.
  Article PubMed CAS Google Scholar
• Chen Y.P., Pettis J.S., Evans J.D., Kramer M., Feldlaufer M.F. (2004) Transmission of Kashmir bee virus by the ectoparasitic mite Varroa destructor, Apidologie 35, 441–448.
  Article Google Scholar
• Chen Y.P., Higgins J.A., Feldlaufer M.F. (2005) Quantitative real-time reverse transcription-PCR analysis of deformed wing virus infection in the honeybee (Apis mellifera L.), Appl. Environ. Microbiol. 71, 436–441.
  Article PubMed CAS Google Scholar
• Chen Y.P., Pettis J.S., Collins A., Feldlaufer M.F. (2006) Prevalence and transmission of honeybee viruses, Appl. Environ. Microbiol. 72, 606–611.
  Article PubMed CAS Google Scholar
• de Miranda J.R., Drebot M., Tyler S., Shen M., Cameron C.E., Stoltz D.B., Camazine S.M. (2004) Complete nucleotide sequence of Kashmir bee virus and comparison with acute bee paralysis virus, J. Gen. Virol. 85, 2263–2270.
  Article PubMed Google Scholar
• Fievet J., Tentcheva D., Gauthier L., de Miranda J.R., Cousserans F., Colin M.E., Bergoin M. (2006) Localization of deformed wing virus infection in queen and drone Apis mellifera L., Virol. J. 3, 1–5.
  Article Google Scholar
• Freeman W.M., Walker S.J., Vrana K.E. (1999) Quantitative RT-PCR: pitfalls and potential, Biotechniques 26, 112–122, 124–125.
  PubMed CAS Google Scholar
• Fujiyuki T., Takeuchi H., Ono M., Ohka S., Sasaki T., Nomoto A., Kubo T. (2004) Novel insect picornalike virus identified in the brains of aggressive worker honeybees, J. Virol. 78, 1093–1100.
  Article PubMed CAS Google Scholar
• Genersch E. (2005) Development of a rapid and sensitive RT-PCR method for the detection of deformed wing virus, a pathogen of the honeybee (Apis mellifera), Vet. J. 169, 121–123.
  Article PubMed CAS Google Scholar
• Ghosh R.C., Ball B.V., Willcocks M.M., Carter M.J. (1999) The nucleotide sequence of sacbrood virus of the honey bee: an insect picorna-like virus, J. Gen. Virol. 80, 1541–1549.
  PubMed CAS Google Scholar
• Govan V.A., Leat N., Allsopp M., Davison S. (2000) Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses, Virology 277, 457–463.
  Article PubMed CAS Google Scholar
• Grabensteiner E., Ritter W., Carter M.J., Davison S., Pechhacker H., Kolodziejek J., Boecking O., Derakhshifar I., Moosbeckhofer R., Licek E., Nowotny N. (2001) Sacbrood virus of the honeybee (Apis mellifera): rapid identification and phylogenetic analysis using reverse transcription-PCR, Clin. Diagn. Lab. Immunol. 8, 93–104.
  PubMed CAS Google Scholar
• Johnson D.R., Lee P.K., Holmes V.F., Alvarez-Cohen L. (2005) An internal reference technique for accurately quantifying specific mRNAs by real-time PCR with application to the tceA reductive dehalogenase gene, Appl. Environ. Microbiol. 71, 3866–3871.
  Article PubMed CAS Google Scholar
• Lanzi G., de Miranda J.R., Boniotti M.B., Cameron C.E., Lavazza A., Capucci L., Camazine S.M., Rossi C. (2006) Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.), J. Virol. 80, 4998–5009.
  Article PubMed CAS Google Scholar
• Leat N., Ball B., Govan V., Davison S. (2000) Analysis of the complete genome sequence of black queen-cell virus, a picorna-like virus of honey bees, J. Gen. Virol. 81, 2111–2119.
  PubMed CAS Google Scholar
• Martin S.J. (2001) The role of Varroa and viral pathogens in the collapse of honeybee colonies: a modelling approach, J. Appl. Ecol. 38, 1082–1093.
  Article Google Scholar
• Nordstrom S. (2003) Distribution of deformed wing virus within honey bee (Apis mellifera) brood cells infested with the ectoparasitic mite Varroa destructor, Exp. Appl. Acarol. 29, 293–302.
  Article PubMed Google Scholar
• Ongus J.R., Peters D., Bonmatin J.M., Bengsch E., Vlak J.M., Van Oers M.M. (2004) Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor, J. Gen. Virol. 85, 3747–3755.
  Article PubMed CAS Google Scholar
• Ribière M., Triboulot C., Mathieu L., Aurières C., Faucon J.P., Pépin M. (2002) Molecular diagnosis of chronic bee paralysis virus infection, Apidologie 33, 339–351.
  Article Google Scholar
• Shen M., Yang X., Cox-Foster D., Cui L. (2005) The role of varroa mites in infections of Kashmir bee virus (KBV) and deformed wing virus (DWV) in honey bees, Virology 342, 141–149.
  Article PubMed CAS Google Scholar
• Stoltz D., Shen X.R., Boggis C., Sisson G. (1995) Molecular diagnosis of Kashmir bee virus infection, J. Apic. Res. 34, 153–160.
  CAS Google Scholar
• Tentcheva D., Gauthier L., Jouve S., Canabady-Rochelle L., Dainat B., Cousserans F., Colin M.E., Ball B.V., Bergoin M. (2004a) Polymerase Chain Reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor, Apidologie 35, 431–439.
  Article CAS Google Scholar
• Tentcheva D., Gauthier L., Zappulla N., Dainat B., Cousserans F., Colin M.E., Bergoin M. (2004b) Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France, Appl. Environ. Microbiol. 70, 7185–7191.
  Article PubMed CAS Google Scholar
• Tentcheva D., Gauthier L., Bagny L., Fievet J., Dainat B., Cousserans F., Colin M.E., Bergoin M. (2006) Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera L. and Varroa destructor, Apidologie 37, 41–50.
  Article CAS Google Scholar
• Yue C., Genersch E. (2005) RT-PCR analysis of Deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor), J. Gen. Virol. 86, 3419–3424.
  Article PubMed CAS Google Scholar

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