Angiogenesis - A putative new approach in glutamine related therapy (original) (raw)

Abstract

Angiogenesis or the generation of new blood vessels, is an important factor regarding the growth of a tumor. Hence, it becomes a necessary parameter of any kind in therapeutic studies. Glutamine is an essential nutrient of tumor tissue and glutamine related therapy involves clearance of circulatory glutamine by glutaminase. So, whether this enzyme has any effect on angiogenesis of a tumor or not becomes an obvious question. To address this question, this study has been carried out with different murine tumor models.The results indicate that purified glutaminase reduces tumor volume as well as restricts the generation of new blood vessels. Glutaminase is effective in the case of solid as well as ascites tumor models. In the case of induced cancer, the host exhibits delayed onset of neoplasia following enzyme treatment and tumor host interactions determine the intensity of the neovascularisation process. Therefore, it can be concluded that this enzyme might be an effective agent against cancer metastasis.

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References

  1. Bossi P, Viale G, Lee AK, et al: Angiogenesis in colorectal tumors: microvessel quantitation in adenomas and carcinomas with clinicopathological correlations. Cancer Res 55:5049–5053, 1995.
    PubMed CAS Google Scholar
  2. Torry RJ, Rongish BJ: Angiogenesis in the uterus: potential regulation and relation to tumor angiogenesis. Amer J Reprod Immunol 27:171–179, 1992.
    CAS Google Scholar
  3. Folkman J, Shing Y: Angiogenesis. J Biol Chem 26:10931–10934, 1992.
    Google Scholar
  4. Goldie H, Felix MD: Growth characteristics of free tumor cells transferred serially in the peritoneal fluid of the mouse. Cancer Res 11:73–81, 1951.
    PubMed CAS Google Scholar
  5. Nagy JA, Morgan ES, Herzberg KT, et al: Pathogenesis of ascites tumor growth: angiogenesis, vascular remodelling and stroma formation in the peritoneal lining. Cancer Res 55:376–385, 1995.
    PubMed CAS Google Scholar
  6. Miguez M, Davel L, Sacerdote de Lustig E: Lymphocyte-Induced Angiogenesis: Correlation with the metastatic incidence of two murine Mammary Adenocarcinomas. Invasion Metast 6:313–320, 1986.
    CAS Google Scholar
  7. Folkman J: What is the role of angiogenesis in metastasis from Cutaneous Melanoma. Eur J Cancer Clin Oncol 23:361–363, 1987.
    Article PubMed CAS Google Scholar
  8. Netland PA, Zetter BR: Tumor-cell interactions with blood vessels during cancer metastasis. Fundamental Aspects of Cancer Dordrecht: Kluwer Academic Publishers. 84–97, 1989.
    Google Scholar
  9. Greenblat M, Shubik P: Tumor Angiogenesis: Transfilter diffusion studies in the hamster by transparent chamber techniques. J Natl Cancer Inst 41:111–124, 1968.
    Google Scholar
  10. Roberts J, Holcenberg JS, Dolowy WC: Antineoplastic activity of highly purified bacterial glutaminase. Nature 227:1136–1137, 1970.
    Article PubMed CAS Google Scholar
  11. Maity P, Chakraborty S, Bhattacharya P, et al: Isolation and purification of phosphate dependent glutaminase from Sacoma-180 tumor and its antineoplastic effects on murine model system. J Exp Clin Cancer Res (communicated).
  12. Murphy ED: Studies in carcinogen induced carcinoma of the cervix in mice. Am J Pathol 29:608, 1953.
    Google Scholar
  13. Murphy ED: Carcinogenesis of the uterine cervix in mice effect of diethylstilbestrol after limited application of 3-methylcholanthrene. J Natl Cancer Inst 27:611–653,1961.
    PubMed CAS Google Scholar
  14. Quesada AR, Sanchez-Jimenez F, Perez-Rodriguez J, et al: Purification of phosphate dependent glutaminase from isolated mitochondria of Ehrlich ascites tumor cells. Biochem J 256:1031–1036, 1988.
    Google Scholar
  15. Lund P: In methods of enzymatic analysis. Verlag Chemie: Weinheim 8:357–369, 1985.
    CAS Google Scholar
  16. Lowry OH, Rosenbrough NJ, Farr AL, et al: Protein measurements with folin phenol reagent. J Biol Chem 193:265–275, 1951
    PubMed CAS Google Scholar
  17. Danielsen T, Rofstad EK: VEGF, bFGF and EGF in the angiogenesis of human melanoma xenografts. Int J Cancer 76:836–841, 1998.
    Article PubMed CAS Google Scholar
  18. Folkman J: What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst 82:4–6, 1990.
    Article PubMed CAS Google Scholar
  19. Burger MM, Folkman J: UICC study group on basic and clinical research: Tumor Angiogenesis. Int J Cancer 56:311–313, 1994.
    Article PubMed CAS Google Scholar
  20. Nagy JA, Brown LF, Senger DR, et al: Pathogenesis of tumor stroma generation: a critical role for leaky blood vessels and fibrin deposition. Biochim Biophys Acta 948:305–326, 1998.
    Google Scholar
  21. Dvorak HF, Harvey VS, Estrella P, et al: Fibrin containing gels induce angigenesis. Lab Invest 57:673–686, 1987.
    PubMed CAS Google Scholar
  22. Neidle A, Waelsch H: J Biol Chem 234:586, 1959.
    PubMed CAS Google Scholar
  23. Matar P, Celoria GC, Font MT, et al: Angiogenesis induced by tumor host interaction in the rat. J Exp Clin Cancer Res 16:249–253, 1997.
    PubMed CAS Google Scholar

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Authors and Affiliations

  1. Department of Metabolic Regulation, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, 700026, Calcutta, India
    Putul Maity, Sunit Chakraborty & Pritha Bhattacharya

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  1. Putul Maity
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  2. Sunit Chakraborty
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  3. Pritha Bhattacharya
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Correspondence toPutul Maity.

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Maity, P., Chakraborty, S. & Bhattacharya, P. Angiogenesis - A putative new approach in glutamine related therapy.Pathol. Oncol. Res. 5, 309–314 (1999). https://doi.org/10.1053/paor.1999.0230

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