Berberine Inhibits Growth of the Breast Cancer Cell Lines MCF-7 and MDA-MB-231 (original) (raw)

Planta Med 2008; 74(1): 39-42
DOI: 10.1055/s-2007-993779

Pharmacology

Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Jong Bin Kim2 , Kyung-Min Lee2 , Eunyoung Ko1 , Wonshik Han1 , Jeong Eon Lee1 , Incheol Shin4 , Ji-Yeon Bae2 , Sangmin Kim3 , Dong-Young Noh1 , 2

• 1Department of Surgery, Seoul National University College of Medicine, Seoul, Korea
• 2Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
• 3Clinical Research Institute, Seoul National University College of Medicine, Seoul, Korea
• 4Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea

Further Information

Publication History

Received: October 19, 2007 Revised: November 25, 2007

Accepted: November 27, 2007

Publication Date:
17 January 2008 (online)

Abstract

The effects of berberine on the behavior of breast tumors have not yet been established. To determine whether this compound is useful in the treatment of breast cancer, we analyzed the impact of berberine on the human breast cancer cell lines MCF-7 and MDA-MB-231 cells. Berberine was added to proliferating MCF-7 and MDA-MB-231 cells in culture. Following treatment, changes in cell growth characteristics such as proliferation, cell cycle duration, and the degree of apoptosis were assayed. Following berberine treatment, a time-dependent reduction in proliferation was observed in both cell lines at differing concentrations: 20 μM for MCF-7 and 10 μM for MDA-MB-231 cells. Annexin V staining showed an increase in apoptosis in both cell lines of 31 % in MCF-7and 12 % in MDA-MB-231 cells compared to their respective controls. In addition, 12 % of the MCF-7 cells were arrested at G0/G1, compared to 62 % of control cells. These results demonstrate that treatment with berberine inhibits growth in both MDA-MB-231 and MCF-7 cells. In addition, they show that this partly occurs through the induction of apoptosis in MDA-MB-231 cells, and through both cell cycle arrest and induction of apoptosis in MCF-7 cells. Thus, berberine may be a novel therapeutic drug for breast cancer.

Key words

Berberine - breast cancer - MCF-7 - MDA-MB-231 - apoptosis

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Dong-Young Noh, M.D., Ph. D.

Cancer Research Institute and Department of Surgery

Seoul National University College of Medicine

28 Yongon-dong

Chongno-gu

Seoul 110-744

Korea

Phone: +82-2-760-2921

Fax: +82-2-766-3975

Email: dynoh@snu.ac.kr