HPLC Separation and Quantitative Determination of Ginsenosides from Panax ginseng, Panax quinquefolium and from Ginseng Drug Preparations (original) (raw)

Planta Med 1980; 39(8): 348-357
DOI: 10.1055/s-2008-1074929

Research Articles

© Georg Thieme Verlag Stuttgart · New York

2nd Communication

• 1Pharmaton Ltd., Research and Development, Lugano-Bioggio, Switzerland
• 2Eidgenössische Technische Hochschule Zürich, Pharmazeutisches Institut, Zürich, Switzerland

Further Information

Publication History

Publication Date:
29 April 2008 (online)

Abstract

A new HPLC-method for separation and quantitative determination of ginsenosides in Panax ginseng, Panax quinquefolium and in pharmaceutical drug preparations is elaborated. A reversed-phase-system with (μBondapak C18 column (3.9 mm I. D. × 30 cm) using acetonitrile-water (30:70) 2 ml/min and acetonitrile-water (18:82) 4 ml/min is suitable for the baseline separation of Rb1; Rb2, Re, Rd, Rf, Rg2, respectively Re, Rg! in 30 minutes. The ginsenosides are directly detected at 203 nm (without derivatization) with the LC-55 or LC-75 spectrophotometer (Perkin-Elmer) at 100 % transmission. Detection limit is 300 ng at a signal-to-noise ratio of 10:1. The calibration curve of each ginsenoside has a correlation coefficient very near to

1. Relative standard deviation for quantitative determinations depends upon the amount of ginsenosides and is approximately 1 % for ginsenoside contents of 1 %. This method is adaptable for routine analysis in quality control laboratories.

Key Word Index

Panax ginseng - Panax quinquefolium - Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2 - Triterpene Saponines - HPLC.