Withanolide Z, a New Chlorinated Withanolide from Withania somnifera (original) (raw)

Planta Med 2008; 74(14): 1745-1748
DOI: 10.1055/s-2008-1081357

Letter

Natural Products Chemistry
© Georg Thieme Verlag KG Stuttgart · New York

Swapan Pramanick1 , Amit Roy2 , Shekher Ghosh1 , Hemanta K. Majumder2 , Sibabrata Mukhopadhyay1

1Medicinal Chemistry Division, Indian Institute of Chemical Biology, Jadavpur, Kolkata, India
2Department of Molecular Parasitology, Indian Institute of Chemical Biology, Jadavpur, Kolkata, India

Further Information

Publication History

Received: March 22, 2008 Revised: July 9, 2008

Accepted: July 11, 2008

Publication Date:
05 November 2008 (online)

Abstract

Reverse-phase preparative HPLC analysis of the _n_-butanol fraction of the methanolic extract of Withania somnifera Dunal (leaves) afforded a novel chlorinated withanolide, namely withanolide Z (1), along with four known withanolides, withanolide B (2), withanolide A (3), 27-hydroxywithanolide B (4) and withaferin A (5). Their structures were elucidated by IR, MS, CD and a combination of 1 D and 2 D NMR spectral analyses. The Leishmania donovani DNA topoisomerase I inhibitory activities of the isolated compounds were determined.

Key words

Solanaceae - Withania somnifera - withanolide - HPLC - Leishmania donovani DNA topoisomerase I

Supporting Information for this article is available online at

Supporting Information .

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Dr. Sibabrata Mukhopadhyay

Medicinal Chemistry Division

Indian Institute of Chemical Biology

4 Raja S.C.Mullick Road

Jadavpur

Kolkata-700 032

India

Phone: +91/2473/3491/0492

Fax: +91/3324/735/197

Email: sbmukh@yahoo.co.uk

Supplementary Material