Fast imaging and fast force spectroscopy of single biopolymers with a new atomic force microscope designed for small cantilevers (original) (raw)
Research Article| November 01 1999
Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 München, Germany
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Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, 80799 München, Germany
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Department of Chemistry, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Department of Physics, University of California at Santa Barbara, Santa Barbara, California 93106
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Rev. Sci. Instrum. 70, 4300–4303 (1999)
Citation
M. B. Viani, T. E. Schäffer, G. T. Paloczi, L. I. Pietrasanta, B. L. Smith, J. B. Thompson, M. Richter, M. Rief, H. E. Gaub, K. W. Plaxco, A. N. Cleland, H. G. Hansma, P. K. Hansma; Fast imaging and fast force spectroscopy of single biopolymers with a new atomic force microscope designed for small cantilevers. _Rev. Sci. Instrum. 1 November 1999; 70 (11): 4300–4303. https://doi.org/10.1063/1.1150069
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Small cantilevers allow for faster imaging and faster force spectroscopy of single biopolymers than previously possible because they have higher resonant frequencies and lower coefficients of viscous damping. We have used a new prototype atomic force microscope with small cantilevers to produce stable tapping-mode images (1 μm×1 μm) in liquid of DNA adsorbed onto mica in as little as 1.7 s per image. We have also used these cantilevers to observe the forced unfolding of individual titin molecules on a time scale an order of magnitude faster than previously reported. These experiments demonstrate that a new generation of atomic force microscopes using small cantilevers will enable us to study biological processes with greater time resolution. Furthermore, these instruments allow us to narrow the gap in time between results from force spectroscopy experiments and molecular dynamics calculations.
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