Molecular characterization of an Enterovirus 71 causing neurological disease in Germany (original) (raw)

Abstract

Enterovirus 71 (EV71) is mainly known as a cause of hand-foot-and-mouth disease (HFMD) but sometimes associated with neurological disease, even as fatal brainstem encephalitis. In Europe, EV71 infections are extremely rare, in contrast to the worldwide situation. This is the first report of molecular characterization of an EV71 strain isolated in Europe that had caused neurological disease. The german strain is closest related to sublineage B2 strains isolated in the United States, which where mainly associated with neurological disease. Phylogenetic analysis also showed that the strain must have been imported to Germany several years ago, and continues to circulate since then.

Access this article

Log in via an institution

Subscribe and save

• Get 10 units per month
• Download Article/Chapter or eBook
• 1 Unit = 1 Article or 1 Chapter
• Cancel anytime Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

• Brown BA, Oberste MS, Alexander JP Jr, Kennett ML, Pallansch MA (1999). Molecular epidemiology and evolution of enterovirus 71 strains isolated from 1970 to 1998. J Virol 73: 9969–9975.
  PubMed CAS Google Scholar
• Chan LG, Parashar UD, Lye MS, Ong FG, Zaki SR, Alexander JP, Ho KK, Han LL, Pallansch MA, Suleiman AB, Jegathesan M, Anderson LJ (2000). Deaths of children during an outbreak of hand, foot and mouth disease in Sarawak, Malaysia: clinical and pathological characteristics of the disease. Clin Infect Dis 31: 678–683.
  Article PubMed CAS Google Scholar
• Chapman NM, Tracy S, Gauntt CJ, Fortmueller U (1990). Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization. J Clin Microbiol 28: 843–850.
  PubMed CAS Google Scholar
• Ho M, Chen ER, Hsu KH, Twu SJ, Chen KT, Tsai SF, Wang JR, Shih SR (1999). An epidemic of enterovirus 71 infection in Taiwan. N Engl J Med 341: 929–935.
  Article PubMed CAS Google Scholar
• Landry ML, Fonseca SN, Cohen S, Bogue CW (1995). Fatal enterovirus type 71 infection: rapid detection and diagnostic pitfalls. Pediatr Infect Dis J 14: 1095–1100.
  Article PubMed CAS Google Scholar
• McMinn P, Lindsay K, Perera D, Chan HM, Chan KP, Cardosa MJ (2001). Phylogenetic analysis of enterovirus 71 strains isolated during linked epidemics in Malaysia, Singapore, and Western Australia. J Virol 75: 7732–7738.
  Article PubMed CAS Google Scholar
• Saitou N, Nei M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol BiolEvol 4: 406–425.
  CAS Google Scholar
• Thompson JD, Higgins DG, Gibson TJ (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap-penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.
  Article PubMed CAS Google Scholar

Download references

Author information

Authors and Affiliations

1. Laboratory G. Enders and Partner and Institute for Virology, Infectology and Epidemiology, e.V., Rosenbergstrasse 85, 70193, Stuttgart, Germany
   Johannes Kehle, Christoph Metzger & Gisela Enders
2. VIROTEST Prof. Enders GmbH, Stuttgart, Germany
   Johannes Kehle, Christoph Metzger & Gisela Enders
3. Department for General Virology, University of Hohenheim, Institute for Genetics, Stuttgart, Germany
   Bernhard Roth & Artur Pfitzner

Authors

1. Johannes Kehle
   You can also search for this author inPubMed Google Scholar
2. Bernhard Roth
   You can also search for this author inPubMed Google Scholar
3. Christoph Metzger
   You can also search for this author inPubMed Google Scholar
4. Artur Pfitzner
   You can also search for this author inPubMed Google Scholar
5. Gisela Enders
   You can also search for this author inPubMed Google Scholar

Corresponding author

Correspondence toJohannes Kehle.

Rights and permissions

About this article

Cite this article

Kehle, J., Roth, B., Metzger, C. et al. Molecular characterization of an Enterovirus 71 causing neurological disease in Germany.Journal of NeuroVirology 9, 126–128 (2003). https://doi.org/10.1080/13550280390173364

Download citation

• Received: 03 June 2002
• Revised: 18 July 2002
• Accepted: 29 August 2002
• Issue Date: January 2003
• DOI: https://doi.org/10.1080/13550280390173364

Keywords