A tumor-associated fibronectin isoform generated by alternative splicing of messenger RNA precursors. (original) (raw)
Article| March 01 1989
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
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Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Search for other works by this author on:
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Search for other works by this author on:
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Search for other works by this author on:
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Search for other works by this author on:
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Search for other works by this author on:
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Search for other works by this author on:
B Carnemolla , E Balza , A Siri , L Zardi , M R Nicotra , A Bigotti , P G Natali
Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1989) 108 (3): 1139–1148.
Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from cultured transformed human cells has a very different isoform composition with respect to its normal counterpart. In fact, SV-40-transformed WI-38VAI3 human fibroblasts produce high levels of a FN isoform (B-FN) which is very poorly expressed in their normal, WI-38, counterpart. We have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in transformed cells, to a high level expression of the exon ED-B (Zardi, L., B. Carnemolla, A. Siri, T. E. Petersen, G. Paolella, G. Sebastio, and F. E. Baralle. 1987. EMBO (Eur. Mol. Biol. Organ.) J. 6:2337-2342). Here we report on the production and characterization of a monoclonal antibody (BC-1) which recognizes an epitope within the protein sequence coded for by the ED-B exon. This monoclonal antibody makes it possible to carry out immunohistochemical analysis of the distribution of the ED-B-containing FN isoform (B-FN) in human tissues. The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. On the contrary, the B-FN isoform has a much greater expression in fetal and tumor tissues. These results demonstrate that, in vivo, different FN isoforms have a differential distribution and indicate that the B-FN isoform may play a role in ontogenesis and oncogenetic processes.
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