Live cell imaging of the assembly, disassembly, and actin cable–dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae | Journal of Cell Biology (original) (raw)
Article| November 08 2004
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
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Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Search for other works by this author on:
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Search for other works by this author on:
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Search for other works by this author on:
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Search for other works by this author on:
Thomas M. Huckaba
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Anna Card Gay
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Luiz Fernando Pantalena
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Hyeong-Cheol Yang
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
Liza A. Pon
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032
H.-C. Yang's present address is Department of Dental Biomaterials, Seoul National University, Seoul 110-749, Korea.
Received: April 29 2004
Accepted: September 14 2004
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 167 (3): 519–530.
Using FM4-64 to label endosomes and Abp1p-GFP or Sac6p-GFP to label actin patches, we find that (1) endosomes colocalize with actin patches as they assemble at the bud cortex; (2) endosomes colocalize with actin patches as they undergo linear, retrograde movement from buds toward mother cells; and (3) actin patches interact with and disassemble at FM4-64–labeled internal compartments. We also show that retrograde flow of actin cables mediates retrograde actin patch movement. An Arp2/3 complex mutation decreases the frequency of cortical, nonlinear actin patch movements, but has no effect on the velocity of linear, retrograde actin patch movement. Rather, linear actin patch movement occurs at the same velocity and direction as the movement of actin cables. Moreover, actin patches require actin cables for retrograde movements and colocalize with actin cables as they undergo retrograde movement. Our studies support a mechanism whereby actin cables serve as “conveyor belts” for retrograde movement and delivery of actin patches/endosomes to FM4-64–labeled internal compartments.
The Rockefeller University Press
2004
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