Impairment of T Cell Development in δEF1 Mutant Mice (original) (raw)
Article| April 21 1997
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Search for other works by this author on:
Yujiro Higashi, Hiroki Moribe, Tsuyoshi Takagi, Ryohei Sekido, Kiyoshi Kawakami, Hitoshi Kikutani, Hisato Kondoh
From the *Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan; ‡Department of Biology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-04, Japan
Address correspondence to Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan.
1 Abbreviations used in this paper: DN, double negative; DP, double positive; d.p.c., days post coitum; FITC, fluorescein isothiocyanate; HBS, Hepes-buffered saline; SP, single positive.
Received: October 29 1996
Revision Received: February 07 1997
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1997) 185 (8): 1467–1480.
Received:
October 29 1996
Revision Received:
February 07 1997
Using the method of gene targeting in mouse embryonic stem cells, regulatory function of δEF1, a zinc finger and homeodomain-containing transcription factor, was investigated in vivo by generating the δEF1 mutant mice. The mutated allele of δEF1 produced a truncated form of the δEF1 protein lacking a zinc finger cluster proximal to COOH terminus. The homozygous δEF1 mutant mice had poorly developed thymi with no distinction of cortex and medulla. Analysis of the mutant thymocyte showed reduction of the total cell number by two orders of magnitude accompanying the impaired thymocyte development. The early stage intrathymic c-kit+ T precursor cells were largely depleted. The following thymocyte development also seemed to be affected as assessed by the distorted composition of CD4- or CD8-expressing cells. The mutant thymocyte showed elevated α4 integrin expression, which might be related to the T cell defect in the mutant mice. In the peripheral lymph node tissue of the mutant mice, the CD4−CD8+ single positive cells were significantly reduced relative to CD4+CD8− single positive cells. In contrast to T cells, other hematopoietic lineages appeared to be normal. The data indicated that δEF1 is involved in regulation of T cell development at multiple stages.
You do not currently have access to this content.
Sign in
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Username (Note: This may be your email address.) ?
Password
Could not validate captcha. Please try again.
5,902 Views
127 Web of Science