Comparative Evaluation of the Cytomegalovirus DNA Load in Polymorphonuclear Leukocytes and Plasma of Human Immunodeficiency Virus-Infected Subjects (original) (raw)

Journal Article

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Centre Hospitalier Universitaire de Québec Research Center in Infectious Diseases and Department of Microbiology, Université Laval, and Hôpital du Saint-Sacrement

,

Québec

;

Hôtel-Dieu de Montréal and Department of Microbiology, Université de Montréal, and Chest Institute and Department of Medicine, McGill University

,

Montréal, Canada

Reprints or correspondence: Dr. Guy Boivin, CHUL, Room RC-709, 2705 Blvd. Laurier, Sainte-Foy, Québec, Canada, G1V 4G2.

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Centre Hospitalier Universitaire de Québec Research Center in Infectious Diseases and Department of Microbiology, Université Laval, and Hôpital du Saint-Sacrement

,

Québec

;

Hôtel-Dieu de Montréal and Department of Microbiology, Université de Montréal, and Chest Institute and Department of Medicine, McGill University

,

Montréal, Canada

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Centre Hospitalier Universitaire de Québec Research Center in Infectious Diseases and Department of Microbiology, Université Laval, and Hôpital du Saint-Sacrement

,

Québec

;

Hôtel-Dieu de Montréal and Department of Microbiology, Université de Montréal, and Chest Institute and Department of Medicine, McGill University

,

Montréal, Canada

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,

Centre Hospitalier Universitaire de Québec Research Center in Infectious Diseases and Department of Microbiology, Université Laval, and Hôpital du Saint-Sacrement

,

Québec

;

Hôtel-Dieu de Montréal and Department of Microbiology, Université de Montréal, and Chest Institute and Department of Medicine, McGill University

,

Montréal, Canada

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,

Centre Hospitalier Universitaire de Québec Research Center in Infectious Diseases and Department of Microbiology, Université Laval, and Hôpital du Saint-Sacrement

,

Québec

;

Hôtel-Dieu de Montréal and Department of Microbiology, Université de Montréal, and Chest Institute and Department of Medicine, McGill University

,

Montréal, Canada

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Centre Hospitalier Universitaire de Québec Research Center in Infectious Diseases and Department of Microbiology, Université Laval, and Hôpital du Saint-Sacrement

,

Québec

;

Hôtel-Dieu de Montréal and Department of Microbiology, Université de Montréal, and Chest Institute and Department of Medicine, McGill University

,

Montréal, Canada

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Revision received:

05 September 1997

Published:

01 February 1998

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Guy Boivin, Julie Handfield, Emil Toma, Gilles Murray, Richard Lalonde, Michel G. Bergeron, Comparative Evaluation of the Cytomegalovirus DNA Load in Polymorphonuclear Leukocytes and Plasma of Human Immunodeficiency Virus-Infected Subjects, The Journal of Infectious Diseases, Volume 177, Issue 2, February 1998, Pages 355–360, https://doi.org/10.1086/514190
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Abstract

The cytomegalovirus (CMV) DNA load was determined in polymorphonuclear leukocytes (PMNL) and plasma samples from 106 human immunodeficiency virus-infected subjects at risk of developing CMV disease (group 1) and from 27 AIDS patients with documented CMV disease (group 2). For both groups, the number of CMV copies in PMNL was significantly higher than in plasma when results were derived from an equivalent blood volume (P < .001, PMNL vs. plasma). Additionally, group 2 (symptomatic) patients had a greater viral DNA load than group 1 (asymptomatic) subjects (P < .001 for both PMNL and plasma). The sensitivity, specificity, and positive and negative predictive values of qualitative polymerase chain reaction using PMNL (PCR-PMNL) for the presence of CMV disease were 100%, 58%, 38%, and 100%, respectively, compared with 70%, 93%, 74%, and 92% for qualitative PCR-plasma and 93%, 92%, 76%, and 98% for quantitative PCRPMNL using a cutoff of 16,000 copies/mL. Thus, the best strategy for diagnosing CMV disease in these individuals relies on quantitative assessment of the viral DNA load in PMNL.

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© 1998 by The University of Chicago

Topic:

• polymerase chain reaction
• acquired immunodeficiency syndrome
• blood volume
• dna
• dna, viral
• immunologic deficiency syndromes
• neutrophils
• plasma
• cytomegalovirus
• viruses

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