Silk fibroin/amniotic membrane 3D bi-layered artificial skin (original) (raw)
Paper
, Ali Samadikuchaksaraei, Alexander M Seifalian, Aleksandra M Urbanska, Hossein Ghanbarian, John G Hardy, Mir Davood Omrani, Masoud Mozafari, Rui L Reis and Subhas C Kundu
Published 20 February 2018 • © 2018 IOP Publishing Ltd
Biomedical Materials,Volume 13,Number 3Citation Mazaher Gholipourmalekabadi et al 2018 Biomed. Mater. 13 035003DOI 10.1088/1748-605X/aa999b
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Dates
- Received 16 March 2017
- Revised 11 October 2017
- Accepted 10 November 2017
- Published 20 February 2018
1748-605X/13/3/035003
Abstract
Burn injuries have been reported to be an important cause of morbidity and mortality and they are still considered as unmet clinical need. Although there is a myriad of effective stem cells that have been suggested for skin regeneration, there is no one ideal scaffold. The aim of this study was to develop a three-dimensional (3D) bi-layer scaffold made of biological decellularized human amniotic membrane (AM) with viscoelastic electrospun nanofibrous silk fibroin (ESF) spun on top. The fabricated 3D bi-layer AM/ESF scaffold was submerged in ethanol to induce _β_-sheet transformation as well as to get a tightly coated and inseparable bi-layer. The biomechanical and biological properties of the 3D bi-layer AM/ESF scaffold were investigated. The results indicate significantly improved mechanical properties of the AM/ESF compared with the AM alone. Both the AM and AM/ESF possess a variety of suitable adhesion cells without detectable cytotoxicity against adipose tissue-derived mesenchymal stem cells (AT-MSCs). The AT-MSCs show increased expression of two main pro-angiogenesis factors, vascular endothelial growth factor and basic fibroblast growth factor, when cultured on the AM/ESF for 7 days, when comparing with AM alone. The results suggest that the AM/ESF scaffold with autologous AT-MSCs has excellent cell adhesion and proliferation along with production of growth factors which serves as a possible application in a clinical setting for skin regeneration.
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