Comparison of bcr-abl Protein Expression and Philadelphia Chromosome Analyses in Chronic Myelogenous Leukemia Patients (original) (raw)

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1From the Department of Molecular Pathology, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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1From the Department of Molecular Pathology, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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2From the Division of Laboratory Medicine, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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2From the Division of Laboratory Medicine, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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4From the Department of Hematology, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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Albert B. Deisseroth, MD, PhD

3From the Department of Bioimmunolherapy, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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1From the Department of Molecular Pathology, The University of Texas, M.D. Anderson Cancer Center, Houston, Texas.

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01 October 1996

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Jie Qiang Guo, Jinying Lian, Armand Glassman, Moshe Talpaz, Hagop Kantarjian, Albert B. Deisseroth, Ralph B. Arlinghaus, Comparison of bcr-abl Protein Expression and Philadelphia Chromosome Analyses in Chronic Myelogenous Leukemia Patients, American Journal of Clinical Pathology, Volume 106, Issue 4, 1 October 1996, Pages 442–448, https://doi.org/10.1093/ajcp/106.4.442
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Abstract

The Philadelphia chromosome (Ph) is found in most chronic myelogenous leukemia (CML) patients. The bcr-abl oncoprotein (P210 or PI85), the product of the fused bcr-abl gene produced by the Ph, is known to be the major factor in initiation and maintenance of the leukemic state in these types of leukemias. The authors have devised a Western blot test to detect and quantitate the bcr-abl oncoprotein in blood and bone marrow cells. The authors analyzed 1,155 peripheral blood samples from CML patients, for which there were same day Ph data, to determine if there was a correlation between bcr-abl protein levels and the percentage of Ph. A ratio of bcr-abl protein (P210 or P185) to normal abl protein (P145), the latter is ubiquitously expressed in all blood cells, has been established as a criterion for quantitating bcr-abl levels. The bcr-abl/abl protein ratio from 399 patient who were 100% Ph-positive had a mean of 2.47 (standard error [SE] of ±0.05); no false negatives were detected. A decreasing ratio was found in patients with de-creasing percentages of Ph. The relationship between the ratio of bcr-abl/abl proteins to the percentage of Ph-positive cells was nearly linear in 392 patients with Ph percentages between 5% to 95% (r = 0.97, P <.001). For patients in remission with no detectable Ph, the bcr-abl/abl ratio had a mean of 0.01 (SE = 0 ± 0.00). The level of bcr-abl expression in samples from peripheral blood and bone marrow also were compared. The results indicated that the amount of bcr-abl protein within peripheral blood is usually similar to that in marrow. In conclusion, quantitation of the bcr-abl oncoprotein in peripheral blood cells or marrow cells as measured by a Western blot assay provides reliable data for both diagnosis and monitoring patients with Ph-chromosomc positive leukemia.

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