In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2 (original) (raw)

Journal Article

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Meloy Laboratories, Inc.

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6715 Electronic Drive, Springfield, Va. 22151

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Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health

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Bethesda, Md. 20014

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Viral Leukemia and Lymphoma Branch, National Cancer Institute, National Institutes of Health

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Bethesda, Md. 20014

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State of California, Department of Public Health

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2151 Berkeley Way, Berkeley, Calif. 94704

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Department of Pathology and Pediatrics, University of Minnesota Medical School

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Minneapolis, Minn. 55455

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Division of Hematology, Jewish Hospital and Medical Center of Brooklyn

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555 Prospect Place, Brooklyn, N.Y. 11238

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Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health

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Bethesda, Md. 20014

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Received:

31 January 1973

Published:

01 November 1973

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Donald J. Giard, Stuart A. Aaronson, George J. Todaro, Paul Arnstein, John H. Kersey, Harvey Dosik, Wade P. Parks, In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors, JNCI: Journal of the National Cancer Institute, Volume 51, Issue 5, November 1973, Pages 1417–1423, https://doi.org/10.1093/jnci/51.5.1417
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Abstract

A series of 200 human tumors were cultivated in vitro in an attempt to establish cell lines. Lines were established, with explant and trypsinization techniques, from 13 tumors including carcinomas, sarcomas, melanomas, and brain tumors. All these lines, In culture for over 1 year, exhibited marked refractility, multilayering, and criss-crossing and were morphologically distinct from normal contact-inhibited human fibroblast or epithelial lines. They also formed colonies on monolayers of normal cells and grew with a high efficiency in soft agar. Preliminary results indicated abnormal chromosomal patterns in all lines tested, and 8 of 9 cell lines formed tumors in antithymo~yte serum-treated mice. The rate of establishment (approximately 6%) of lines from random neoplastic material demonstrated that cells with properties of transformed cells could be recovered from tumor tissue, but it also emphasized the need for improved methodology in this area.

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Topic:

• brain tumors
• carcinoma
• epithelium
• fibroblasts
• agar
• cell lines
• chromosomes
• melanoma
• sarcoma
• mice
• neoplasms
• solid tumors

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