Association of p53 Protein Expression With Tumor Cell Proliferation Rate and Clinical Outcome in Node-Negative Breast Cancer (original) (raw)

Journal Article

,

Department of Pathology, University of Texas Health Science Center

San Antonio

Correspondence to : D. Craig Allred, M.D., Department of Pathology, University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, TX 78284.

Search for other works by this author on:

,

Department of Medicine/Oncology), University of Texas Health Science Center

San Antonio

Search for other works by this author on:

,

Department of Medicine/Oncology), University of Texas Health Science Center

San Antonio

Search for other works by this author on:

,

Department of Medicine/Oncology), University of Texas Health Science Center

San Antonio

Search for other works by this author on:

,

Department of Pathology, Baylor College of Medicine

Houston, Tex.

Search for other works by this author on:

,

Department of Medicine/Oncology), University of Texas Health Science Center

San Antonio

Search for other works by this author on:

,

Department of Medicine/Oncology), University of Texas Health Science Center

San Antonio

Search for other works by this author on:

Department of Medicine/Oncology), University of Texas Health Science Center

San Antonio

W. L. McGuire is deceased

Search for other works by this author on:

Revision received:

23 October 1992

Accepted:

30 October 1992

Published:

03 February 1993

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

D. Craig Allred, Gary M. Clark, Richard Elledge, Suzanne A. W. Fuqua, Richard W. Brown, Gary C. Chamness, C. Kent Osborne, William L. McGuire, Association of p53 Protein Expression With Tumor Cell Proliferation Rate and Clinical Outcome in Node-Negative Breast Cancer, JNCI: Journal of the National Cancer Institute, Volume 85, Issue 3, 3 February 1993, Pages 200–206, https://doi.org/10.1093/jnci/85.3.200
Close

Navbar Search Filter Mobile Enter search term Search

Abstract

Background : The p53 (also known as TP53) tumor suppressor gene encodes for a nuclear phosphoprotein thought to regulate proliferation of normal cells. Most p53 mutations result in a nonfunctional protein that accumulates in tumor cell nuclei. These common mutations appear to be involved in the development and/or progression of several neoplastic diseases including human breast cancer. Purpose : Our purpose was to investigate the relationships between levels of mutant p53 protein expression, tumor cell proliferation rate, and clinical outcome in patients with node-negative breast cancer. Methods : Expression of mutant p53 protein was evaluated by frozen-section immunohistochemistry (IHC) and light microscopy in 700 breast cancers from axillary lymph node-negative patients with long-term follow-up (median, 54 months). The immunostaining signal was expressed as the sum of scores representing the proportion and staining intensity of negative and positive tumor cell nuclei (ranges, 0 and 2–8, respectively). Statistical comparisons were made between levels of p53 protein expression and disease-free survival, overall survival, and tumor proliferation rate expressed as the percentage of cells in the S phase (%S phase) as determined by flow cytometry. Results : Of the 700 tumors, 362 (52%) showed positive nuclear immunostaining (IHC score >0). Proliferation rates were significantly higher ( P = .0001) in positive tumors (median %S phase, 7.1%) than in negative tumors (4.1%). In a univariate cutpoint analysis, negative tumors (n = 388) versus low-positive tumors (IHC score = 2–6; n = 263) versus high-positive tumors (IHC score >6; n = 99) showed progressively reduced disease-free survival (80% versus 72% versus 58% at 5 years, respectively; P ≤.05 for all pairwise comparisons). Analogous results for overall survival were 88% versus 84% versus 74%; only the result for negative versus high positive tumors was significant ( P = .003). In a multivariate analysis, expression of p53 protein and high %S phase were independently associated with reduced disease-free survival ( P = .008 and .01, respectively). Conclusions : Expression of mutant p53 protein was associated with high tumor proliferation rate, early disease recurrence, and early death in node-negative breast cancer. Despite the strong direct correlation between accumulation of p53 protein and tumor proliferation rate, both factors were independently associated with poor prognosis, suggesting that p53 may have other biological functions in addition to cell-cycle regulation. Implications : This test, when combined with other prognostic factors, may enhance our ability to identify node-negative breast cancer patients at high risk for early disease recurrence and/or death, for whom the use of adjuvant chemotherapy is unequivocally justified. [J Natl Cancer Inst 85:200–206, 1993]

This content is only available as a PDF.

© Oxford University Press

Topic:

• immunohistochemistry
• mutation
• cell cycle
• cell proliferation
• cancer
• adjuvant chemotherapy
• flow cytometry
• frozen sections
• axilla
• cell nucleus
• follow-up
• tumor suppressor genes
• lymph
• phosphoproteins
• protein p53
• s phase
• neoplasms
• treatment outcome
• breast cancer
• prognostic factors
• light microscopy

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Association of p53 Protein Expression With Tumor Cell Proliferation Rate and Clinical Outcome in Node-Negative Breast Cancer - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 410

187 Pageviews

223 PDF Downloads

Since 11/1/2016

Citations

763 Web of Science

×

Email alerts

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic