Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29 (original) (raw)
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Departments of Biochemistry and Cell Biology, Albert Einstein College of Medicine
1300 Morris Park Avenue, Bronx, NY 10461, USA
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Departments of Biochemistry and Cell Biology, Albert Einstein College of Medicine
1300 Morris Park Avenue, Bronx, NY 10461, USA
Search for other works by this author on:
Received:
24 February 1982
Revision received:
19 April 1982
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Howard M. Fried, Jonathan R. Warner, Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29, Nucleic Acids Research, Volume 10, Issue 10, 25 May 1982, Pages 3133–3148, https://doi.org/10.1093/nar/10.10.3133
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Abstract
A cosmid clone bank of yeast DNA has been used to isolate the cycloheximide resistance gene cyh2 of Saccharomyces cerevisiae. A cosmid carrying this gene was identified by cross hybridization to another cloned gene, tsm437. The two genes, which are tightly linked genetically are both present on a 31 kb segment of cloned DNA. The cyh2 gene encodes ribosomal protein L29, a component of the large subunit. Blot hybridization analysis reveals that this gene is present as a single copy in the yeast genome, unlike many other yeast ribosomal protein genes which appear to be duplicated. The cyh2 gene also appears to contain an intervening sequence, a characteristic common to most yeast ribosomal protein genes that have been cloned.
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