Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX (original) (raw)

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Transgène S.A., 11 rue Humann, 67000 Strasbourg, France

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Received:

22 February 1983

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Michael Jaye, Henri de la Salle, Fabienne Schamber, Alain Balland, Vipin Kohli, Annie Findeli, Paul Tolstoshev, Jean-Pierre Lecocq, Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX, Nucleic Acids Research, Volume 11, Issue 8, 25 April 1983, Pages 2325–2335, https://doi.org/10.1093/nar/11.8.2325
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Abstract

A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.

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