A role for exon sequences in alternative splicing of the human fibronection gene (original) (raw)

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Sir William Dunn School of Pathology, University of Oxford

South Parks Road, Oxford OX1 3RE, UK

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Sir William Dunn School of Pathology, University of Oxford

South Parks Road, Oxford OX1 3RE, UK

*Present address: Department of Paediatrics, 2nd School of Medicine, via S.Pansini 5, Naples 80131, Italy

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Sir William Dunn School of Pathology, University of Oxford

South Parks Road, Oxford OX1 3RE, UK

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Accepted:

04 September 1987

Published:

12 October 1987

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Helen J. Mardon, Gianfranco Sebastio, Francisco E. Baralle, A role for exon sequences in alternative splicing of the human fibronection gene, Nucleic Acids Research, Volume 15, Issue 19, 12 October 1987, Pages 7725–7733, https://doi.org/10.1093/nar/15.19.7725
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Abstract

Exon EDIIIA of the fibronectin (Fn) gene is alternatively spliced via pathways which either skip or include the whole exon in the messenger RNA (mRNA). We have investigated the role of EDIIIA exon sequences in the human Fn gene in determining alternative splicing of this exon during transient expression of α globin/Fn minigene hybrids in HeLa cells. We demonstrate that a DNA sequence of 81bp within the central region of exon EDIIIA is required for alternative splicing during processing of the primary transcript to generate both EDIIIA+ and EDIIIA− mRNA's. Furthermore, alternative splicing of EDIIIA only occurs when this sequence is present in the correct orientation since when it is in antisense orientation splicing always occurs via exon-skipping generating EDIIIA− mRNA.

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