Dual enhancer activities of the cyclic-AMP responsive element with cell type and promoter specificity (original) (raw)

Journal Article

,

Laboratory of Molecular Genetics, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN)

Tsukuba, Ibaraki 305, Japan

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Laboratory of Molecular Genetics, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN)

Tsukuba, Ibaraki 305, Japan

Search for other works by this author on:

Received:

22 November 1988

Accepted:

03 January 1989

Published:

25 February 1989

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Chie Kanei-Ishii, Shunsuke Ishii, Dual enhancer activities of the cyclic-AMP responsive element with cell type and promoter specificity, Nucleic Acids Research, Volume 17, Issue 4, 25 February 1989, Pages 1521–1536, https://doi.org/10.1093/nar/17.4.1521
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Abstract

The role of the cyclic-AMP (cAMP) responsive element (CRE) in eukaryotic gene transcription was investigated in several cell lines transfected by constructs containing the chloramphenicol acetyltransferase (CAT) gene linked to the three different promoters, simian virus(SV) 40, human c-Ha-ras-1, or chicken β-actin promoter, with or without CRE. CRE had inducible enhancer activity only when it was linked to the SV40 promoter and in a few cell lines such as PC12. CRE functioned as a constitutive enhancer with the human c-Ha-ras-1 promoter in all cell lines examined. CRE also had constitutive enhancer activity when it was linked to the chickenβ-actin promoter, but this activity was observed only in KB, HeLa, and A431 cells. The different types of enhancer activities of CRE depending on the cell and promoter may be caused by interaction with different trans-acting factors that were demonstrated by gel retardation analyses.

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