Genomic fingerprints produced by PCR with consensus tRNA gene primers (original) (raw)
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California Institute of Biological Research
11099 North Torrey Pines Rd, La Jolla, CA 92037, USA
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Received:
18 October 1990
Revision received:
14 December 1990
Accepted:
14 December 1990
Published:
25 February 1991
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John Welsh, Michael McClelland, Genomic fingerprints produced by PCR with consensus tRNA gene primers, Nucleic Acids Research, Volume 19, Issue 4, 25 February 1991, Pages 861–866, https://doi.org/10.1093/nar/19.4.861
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Abstract
The polymerase chain reaction using only a single ‘consensus’ tRNA gene primer, or a pair of primers facing outward from tRNA genes, amplifies a set of DNA fragments in bacterial, plant and animal genomlc DNAs. Presumably, these PCR fingerprints are mainly derived from the regions between closely linked tRNA genes. The pattern of the PCR products is determined by which genomes and which primer(s) are used. Genomic fingerprints are largely conserved within a species and, in bacteria, most products in the fingerprint are conserved between closely related species. Thus, PCR with tRNA gene consensus primers helps to identify specles and genera.
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