A mechanism for deletion formation in DNA by human cell extracts: the involvement of short sequence repeats (original) (raw)

Journal Article

,

MRC Radiobiology Unit

Chilton, Didcot, Oxon OX11 0RD, UK

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,

MRC Radiobiology Unit

Chilton, Didcot, Oxon OX11 0RD, UK

Search for other works by this author on:

,

MRC Radiobiology Unit

Chilton, Didcot, Oxon OX11 0RD, UK

Search for other works by this author on:

MRC Radiobiology Unit

Chilton, Didcot, Oxon OX11 0RD, UK

Search for other works by this author on:

Received:

13 October 1992

Revision received:

05 November 1992

Accepted:

05 November 1992

Published:

11 December 1992

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John Thacker, Jeremy Chalk, Anil Ganesh, Phillip North, A mechanism for deletion formation in DNA by human cell extracts: the involvement of short sequence repeats, Nucleic Acids Research, Volume 20, Issue 23, 11 December 1992, Pages 6183–6188, https://doi.org/10.1093/nar/20.23.6183
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Abstract

DNA molecules carrying a site-specific double-strand break were exposed to nuclear extracts from human cell lines. It was shown previously that breaks could be rejoined correctly by human extracts, but that a proportion of the rejoined molecules had suffered deletions and insertions. The ‘mis-rejoined’ proportion was higher with cell extracts from an individual with the disorder ataxia-telanglectasia than with normal cell extracts. We now show by sequence analysis that deletions in extract-treated molecules occur exclusively between short direct repeats (2–6 base pairs). A misrejoined molecule containing an insertion of 300 bp also had a repeat-based deletion at the same site. A number of different direct repeats are involved; however, some clustering of these occurs especially on the upstream side of the initial breakpoint. These data are most simply interpreted in terms of a model of deletion formation involving single-strand exposure and repair, perhaps with the action of other DNA-metabolising enzymes influencing the frequency with which some repeats are involved.

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© 1992 Oxford University Press

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