Specific transcriptional activation in vitro by the herpes simplex virus protein VP16 (original) (raw)
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- Present address: University of California, San Diego, Department of Biology, 0322, 9500 Gilman Drive, La Jolla, CA 92093-0322, USA
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MRC Virology Unit
Church Street, Glasgow G11 5JR, UK
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MRC Virology Unit
Church Street, Glasgow G11 5JR, UK
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1
MRC Virology Unit
Church Street, Glasgow G11 5JR, UK
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MRC Virology Unit
Church Street, Glasgow G11 5JR, UK
* To whom correspondence should be addressed
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- Present address: University of California, San Diego, Department of Biology, 0322, 9500 Gilman Drive, La Jolla, CA 92093-0322, USA
Received:
23 September 1993
Revision received:
08 November 1993
Accepted:
08 November 1993
Published:
11 December 1993
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David N. Arnosti, Chris M. Preston, Michael Hagmann, Walter Schaffner, R.Graham Hope, Gerard Laughlan, Ben F. Luisi, Specific transcriptional activation in vitro by the herpes simplex virus protein VP16, Nucleic Acids Research, Volume 21, Issue 24, 11 December 1993, Pages 5570–5576, https://doi.org/10.1093/nar/21.24.5570
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Abstract
The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, fulllength VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1&2.urule;POU) to a HeLa cell in vitro transcription system. Stimulation of transcription was dependent on the IE-specific element, TAATGARAT. In agreement with earlier observations from electrophoretic mobility shift assays, activation was not observed when Oct-2&2.urule;POU, the DNA-binding domain from the Oct-2 protein, was substituted for Oct-1&2.urule;POU. Single round transcription assays revealed that, together, VP16 and Oct-1&2.urule;POU facilitate the assembly of pre-initiation complexes at target gene promoters.
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Author notes
- Present address: University of California, San Diego, Department of Biology, 0322, 9500 Gilman Drive, La Jolla, CA 92093-0322, USA
© 1993 Oxford University Press
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