U24, a novel intron-encoded small nucleolar RNA with two 12 nt long, phylogenetically conserved complementaritiesm to 28S rRNA (original) (raw)

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Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

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Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

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Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

Search for other works by this author on:

,

Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

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,

Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

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Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

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Michele Caizergues-Ferrer

Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

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Jean-Pierre Bachellerie*

Laborataire de Biologie Moléculaire Eucaryote du CNRS, Université Paul-Sabatier

118 route de Narbonne 31062 Toulouse Cèdex, France

*To whom correspondence should be addressed

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Liang-Hu Qu, Yves Henry, Monique Nicoloso, Bernard Michot, Marie-Claude Azum, Marie-Hélène Renalier, Michele Caizergues-Ferrer, Jean-Pierre Bachellerie, U24, a novel intron-encoded small nucleolar RNA with two 12 nt long, phylogenetically conserved complementaritiesm to 28S rRNA, Nucleic Acids Research, Volume 23, Issue 14, 25 July 1995, Pages 2669–2676, https://doi.org/10.1093/nar/23.14.2669
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Abstract

Following computer searches of sequence banks, we have positively identified a novel Intronlc snoRNA, U24, encoded in the ribosomal protein L7a gene in humans and chicken. Like previously reported intronic snoRNAs, U24 is devoid of a 5-trimethyl-cap. U24 is immunoprecipitated by an antifibrillarin antibody and displays an exclusively nucleolar localization by fluorescence microscopy after in situ hybridization with antisense ollgonucleotldes. In vertebrates, U24 is a 76 nt long conserved RNA which Is metabolically stable, present at ∼14 000 molecules per human HeLa cell. U24 exhibits a 5′–3′ terminal stem-box C-box D structure,typical for several snoRNAs, and contains two 12 nt long conserved sequences complementary to 28SrRNA. It is, therefore, strikingly related to U14, U20 and U21 snoRNAs which also possess long sequences complementary to conserved sequences of mature 18S or 28S rRNAs. In 28S rRNA the two tracts complementary to U24 are adjacent to each other, they involve several methylated nucleotides and are surprisingly close, within the rRNA secondary structure, to complementarities to snoRNAs U18 and U21. Identification of the yeast Saccharomyces cerevislae U24 gene directly confirms the outstanding conservation of the complementarity to 28S rRNA during evolution, suggesting a key role of U24 pairing to pre-rRNA during ribosome biogenesis, possibly in the control of pre-rRNA folding. Yeast S.cerevislae U24 isalso intron-encoded but not In the same host-gene as in humans or chicken.

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