The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. (original) (raw)
Journal Article
,
College of Agricultural and Life Sciences, Department of Biochemistry and Genetics, University of Wisconsin-Madison
420 Henry Mall, Madison, WI 53706, USA.
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,
College of Agricultural and Life Sciences, Department of Biochemistry and Genetics, University of Wisconsin-Madison
420 Henry Mall, Madison, WI 53706, USA.
Search for other works by this author on:
College of Agricultural and Life Sciences, Department of Biochemistry and Genetics, University of Wisconsin-Madison
420 Henry Mall, Madison, WI 53706, USA.
Search for other works by this author on:
Received:
12 November 1975
Published:
01 February 1976
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David L. Smith, Frederick R. Blattner, Julian Davies, The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii., Nucleic Acids Research, Volume 3, Issue 2, 1 February 1976, Pages 343–354, https://doi.org/10.1093/nar/3.2.343
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Summary
We describe the isolation of a new class 2 restriction endonudease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires mg$ $ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.
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