Chromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence (original) (raw)

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Developmental Biochemistry Section, Laboratory of Nutrition and Endocrinology, NIAMDD National Institutes of Health

Bethesda, MD 20014, USA

* Address correspondence to: Dr. Robert T.Simpson, Bldg. 6, Rm. Bl-38, National Institutes of Health, Bethesda, MD 20014, USA

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Developmental Biochemistry Section, Laboratory of Nutrition and Endocrinology, NIAMDD National Institutes of Health

Bethesda, MD 20014, USA

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Received:

04 January 1979

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Robert T. Simpson, Peter Künzler, Chromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence, Nucleic Acids Research, Volume 6, Issue 4, 1 April 1979, Pages 1387–1415, https://doi.org/10.1093/nar/6.4.1387
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Abstract

Chicken erythrocyte inner histones (H2A, H2B, H3 and H4) were associated with the two complementary homopolymeric polydeoxyribonucleotides and the two alternating copolymeric polydeoxyribonucleotides. No evidence for formation of chromatin-like structures was obtained for the complexes with poly(dG)⋅poly(dC) or poly(dA)⋅poly(dT). Both poly(dGdC)⋅poly(dGdC) and poly(dAdT)⋅poly(dAdT) could be folded by histones to yield material digested by DNAase I to multiples of about 10 and by staphylococcal nuclease to 146 bp core particles. Due to the lack of sequence heterogeniety in the complex of histones with poly(dAdT)⋅poly(dAdT), core particles with remarkable fine structural detail are obtained. The internal organization of DNA in the AT-containing and GC-containing core particles appears not to be identical.

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