Evidence that populations of Dictyostelium single-copy mRNA transcripts carry common repeat sequences (original) (raw)

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Department of Biology (B-022), University of California

San Diego, La Jolla, CA 92093, USA

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Department of Biology (B-022), University of California

San Diego, La Jolla, CA 92093, USA

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Karen L. Kindle, Richard A. Firtel, Evidence that populations of Dictyostelium single-copy mRNA transcripts carry common repeat sequences, Nucleic Acids Research, Volume 6, Issue 7, 11 June 1979, Pages 2403–2422, https://doi.org/10.1093/nar/6.7.2403
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Abstract

Two recombinant plasmids, M4 and KH10, carrying Dictyostelium DNA inserted into the Eco RI restriction endonuclease site of pMB9 by poly(dA)-poly(dT) tailing, were selected for study because they are complementary to abundant mRNA populations from Dictyostelium . Both plasmids have been shown to hybridize a heterogeneous size class of mRNAs which, in the case of KN10, comprise 5–10% of the pulse-labeled poly(A) + RNA from vegetative cells. Analysis of the sequence organization of the two pieces of Dictyostelium DNA shows that they consist mostly of single-copy sequences with a short DNA sequence which is repeated in the genome and interspersed with single-copy DNA. These and other results suggest that the majority of the hybridization of pulse-labeled mRNA to M4 and KH10 is to the short “repeated” DNA sequences. In the genome, members of these repeat families appear to be transcribed onto a population of different single-copy mRNAs. Additional results show that M4 DNA contains a sequence which is entirely complementary to a discrete mRNA.

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