The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe (original) (raw)
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* Present address: Institute of Organic Chemistry, Polish Academy of Sciences, 61 704 Poznan, Noskowskiego 12/14, Poland
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Department of Biological Sciences, University of Missouri
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* Present address: Institute of Organic Chemistry, Polish Academy of Sciences, 61 704 Poznan, Noskowskiego 12/14, Poland
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Antoni Rafalski, Jürg Kohli, Paul Agris, Dieter Söll, The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe, Nucleic Acids Research, Volume 6, Issue 8, 25 June 1979, Pages 2683–2695, https://doi.org/10.1093/nar/6.8.2683
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Abstract
The UGA suppressor tRNA produced by Schizosaccharomyces pombe strain sup3-e was purified to homogeneity. It can be aminoacylated with serine by a crude aminoacyl-tRNA synthetase preparation from S. pombe cells. By combining post-labeling fingerprinting and gel sequencing methods the nucleotide sequence of this tRNA was determined to be: pG-U-C-A-C-U-A-U-G-U-C-ac 4 C-G-A-G-D-G-G-D-D-A-A-G-G-A-m 2 G-ψ-U-A-G-A-N-U-U * -C-A-i 6 A-A-ψ-C-U-A-A-U-G-G-G-C-U-U-U-G-C-C-C-G-m 5 C-G-C-A-G-G-T-ψ-C-A-m 1 A-A-U-C-C-U-G-C-U-G-G-U-G-A-C-G-C-C-A OH . The anticodon sequence U * CA is complementary to the UGA codon.
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Author notes
* Present address: Institute of Organic Chemistry, Polish Academy of Sciences, 61 704 Poznan, Noskowskiego 12/14, Poland
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