Ethidium fluorescence assays. Part 1. Physicochemical studies (original) (raw)
Journal Article
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Department of Biochemistry, University of Alberta
Edmonton, Alberta, Canada T6G 2H7
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,
Department of Biochemistry, University of Alberta
Edmonton, Alberta, Canada T6G 2H7
Search for other works by this author on:
,
Department of Biochemistry, University of Alberta
Edmonton, Alberta, Canada T6G 2H7
Search for other works by this author on:
,
Department of Biochemistry, University of Alberta
Edmonton, Alberta, Canada T6G 2H7
Search for other works by this author on:
Department of Biochemistry, University of Alberta
Edmonton, Alberta, Canada T6G 2H7
Search for other works by this author on:
Published:
10 October 1979
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A.R. Morgan, J.S. Lee, D.E. Pulleyblank, N.L. Murray, D.H. Evans, Ethidium fluorescence assays. Part 1. Physicochemical studies, Nucleic Acids Research, Volume 7, Issue 3, 10 October 1979, Pages 547–565, https://doi.org/10.1093/nar/7.3.547
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Abstract
DNA and RNA can be assayed rapidly and very sensitively by exploiting the enhanced fluorescence of ethidium intercalated into duplex regions. By assaying at different pHs and introducing a heating/cooling cycle, a great many physicochemical aspects of DNA and RNA can be studied avoiding the use of radiolabels, and often giving information not otherwise readily obtainable. Studies are described on duplex DNA which involve measurement of extinction coefficients, cross-linking by chemicals, Cot curve analysis as well as estimation of drug-DNA binding constants. The assays can be adapted to investigate multi-stranded nucleic acid structures. The use of co-valently closed circular DNA also allows rapid and extremely sensitive measurements of nicking caused by irradiation or drugs.
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