Synthesis of oligonucleotides on cellulose by a phosphotriester method (original) (raw)
Journal Article
Department of Organic Chemistry
Genentech, Inc., 460 Point San Bruno Blvd., South San Francisco, CA 94080, USA
Search for other works by this author on:
Department of Organic Chemistry
Genentech, Inc., 460 Point San Bruno Blvd., South San Francisco, CA 94080, USA
Search for other works by this author on:
Received:
22 January 1980
PDF Navbar Search Filter Mobile Enter search term Search
Abstract
The synthesis of oligothymidilic acids, (dT) m (where m = 4, 7, 10, 13, 16, 19, 22, and 25), was carried out using a solid phase approach in combination with the modified phosphotriester methodology developed in solution. Cellulose was used as the solid support after its functionalization with a specially featured dinucleoside diphosphate, 5′-O-p-chlorophenylphospho-2′(3′)-0-acetyluridilyl-[2′(3′)-3′]-5′-0-dimethoxytritylthymidine p-chloro-phenylester. The fully protected trideoxynucleoside triphosphste containing only thymidine was repeatedly used to elongate the oligonucleotide chain in the 3′-5′ direction. Individual coupling yields ranged from 45% to 75%. The total time needed to prepare (dT) 25 was four days. Similarly, the tridecanucleotide d(AGAAGGTACTTTT) was synthesized in good yield. The results show that this approach can be used for a fast and economic way to synthesize oligodeoxynucleotides.
This content is only available as a PDF.
© 1980 IRL Press Limited
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.