Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11 (original) (raw)

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Department of Chemical Immunology, The Weizmann Institute of Science

Rehovot, Israel

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Department of Chemical Immunology, The Weizmann Institute of Science

Rehovot, Israel

Search for other works by this author on:

Department of Chemical Immunology, The Weizmann Institute of Science

Rehovot, Israel

Search for other works by this author on:

Published:

25 August 1980

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Rina Zakut, Justus Cohen, David Givol, Cloning and sequence of the cDNA corresponding to the variable region of immunoglobulin heavy chain MPC11, Nucleic Acids Research, Volume 8, Issue 16, 25 August 1980, Pages 3591–3602, https://doi.org/10.1093/nar/8.16.3591
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Abstract

Poly(A)-containing mRNA from mouse myeloma MPC11 was transcribed into cDNA which was cloned in the PstI site of the plasmid pBR322. The transformants were screened by hybridization with a cDNA fragment, derived from plasmid py(11)7 corresponding to the 5′ portion of the constant region of MPC11 heavy chain. Several positive transfonnants were found to contain various lengths of the variable region of the heavy chain We describe the structure and sequence of one of these clones, pV(11)2, which contains cDNA corresponding to the entire variable region of MPC11 heavy chain and extends to codon 248 in the constant region. The protein sequence deduced from the DNA sequence indicates that the variable region of MPC11 heavy chain contains 121 amino acids and belongs to subgroup II of mouse heavy chains. Comparison of this sequence with other heavy chain sequences suggests a J (joining) segment of 16 residues which overlaps five residues of the third hypervariable region. The cDNA sequence shows that there is no discontinuity between the end of the variable region and the beginning of the constant region.

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© 1980 IRL Press Limited

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