Overexpression of Aldehyde Reductase Protects PC12 Cells from the Cytotoxicity of Methylglyoxal or 3-Deoxyglucosone1 (original) (raw)

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Department of Biochemistry, Osaka University Medical School

2-2, Yamadaoka, Suita 565-0871

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Department of Biochemistry, Osaka University Medical School

2-2, Yamadaoka, Suita 565-0871

Search for other works by this author on:

,

Department of Biochemistry, Osaka University Medical School

2-2, Yamadaoka, Suita 565-0871

Search for other works by this author on:

,

Department of Biochemistry, Osaka University Medical School

2-2, Yamadaoka, Suita 565-0871

Search for other works by this author on:

Department of Biochemistry, Osaka University Medical School

2-2, Yamadaoka, Suita 565-0871

Search for other works by this author on:

Received:

20 October 1997

Published:

01 February 1998

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Keiichiro Suzuki, Young Ho Koh, Hisako Mizuno, Rieko Hamaoka, Naoyuki Taniguchi, Overexpression of Aldehyde Reductase Protects PC12 Cells from the Cytotoxicity of Methylglyoxal or 3-Deoxyglucosone, The Journal of Biochemistry, Volume 123, Issue 2, February 1998, Pages 353–357, https://doi.org/10.1093/oxfordjournals.jbchem.a021944
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Abstract

The glycation reaction (Maillard reaction) plays a major role in diabetic complications, since some reaction intermediates are responsible for the modification and cross-linking of long-lived proteins, resulting, in turn, in a deterioration of normal cell function. The reaction intermediates include methylglyoxal (MG) and 3-deoxyglucosone (3-DG), both of which are cytotoxic dicarbonyl compounds and are elevated during hyperglycemia. Aldehyde reductase (ALR) catalyzes the reduction of both compounds. To examine the intracellular role of ALR in the diabetic complications of neural cells, its gene was overexpressed in rat pheochromocytoma PC12 cells, which normally express a low level of ALR. Western blot analysis showed that ALR protein in the ALR gene-transfected cells was more than twice as much as in the control cells. In the parental cells, cytotoxicity, including apoptotic cell death, which was determined by fluorescent microscopy using the fluorescent DNA binding dye Hoechst 33258, was observed at 100 uM MG. In the ALR gene-transfected cells, the cytotoxicity of both MG and 3-DG and apoptotic cell death were decreased. This suggests that intracellular ALR protects neural cells from the cytotoxicity of 3-DG or MG, and that neural cells, which normally express a low level of ALR, might be susceptible to diabetic complications caused by intermediate products of the Maillard reaction, such as 3-DG and MG.

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© 1998 by The Japanese Biochemical Society

Topic:

• pheochromocytoma
• hyperglycemia
• maillard reaction
• western blotting
• cell function
• aldehyde reductase
• cell death
• dna
• dyes
• genes
• neurons
• parent
• pc12 cells
• pyruvaldehyde
• rats
• cytotoxicity
• protein overexpression
• glycation
• cross-linking
• diabetes mellitus complications

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