Toxicity of Free Proline Revealed in an Arabidopsis T-DNA-Tagged Mutant Deficient in Proline Dehydrogenase (original) (raw)

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1_Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1, Koyadai, Tsukuba, Ibaraki, 305-0074 Japan_

2_Genesis Research Institute, Inc., 4-1-35, Noritake-Shinmachi Nishi-Ku, Nagoya, Aichi, 451-0051 Japan_

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3_Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 1-7-22, Suehiro-cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045 Japan_

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3_Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 1-7-22, Suehiro-cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045 Japan_

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4_Kazusa DNA Research Institute, 1532-3, Yana, Kisarazu, Chiba, 292-0812 Japan_

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4_Kazusa DNA Research Institute, 1532-3, Yana, Kisarazu, Chiba, 292-0812 Japan_

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1_Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1, Koyadai, Tsukuba, Ibaraki, 305-0074 Japan_

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Tokihiko Nanjo, Miki Fujita, Motoaki Seki, Tomohiko Kato, Satoshi Tabata, Kazuo Shinozaki, Toxicity of Free Proline Revealed in an Arabidopsis T-DNA-Tagged Mutant Deficient in Proline Dehydrogenase, Plant and Cell Physiology, Volume 44, Issue 5, 15 May 2003, Pages 541–548, https://doi.org/10.1093/pcp/pcg066
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Abstract

The toxicity of proline (Pro) to plant growth has raised questions despite its protective functions in response to environmental stresses. To evaluate Pro toxicity, we isolated an Arabidopsis T-DNA-tagged mutant, pdh, that had a defect in Pro dehydrogenase (AtProDH), which catalyzes the first step of Pro catabolism. The pdh mutant showed hypersensitivity to exogenous application of ≤10 mM l-Pro, at which wild-type plants grew normally. A dose-dependent increase in internal free Pro accumulation was observed in pdh plants during external Pro supply. These results do not just prove the toxicity of Pro, but also suggest that AtProDH is the only enzyme acting as a functional ProDH in Arabidopsis. To further analyze the targets of Pro toxicity, we compared the expression of thousands of genes by pdh plants with that by wild-type plants by cDNA microarray analysis. Most genes were unaffected. Here we demonstrate Pro toxicity by using the pdh mutant and discuss a cause-and-effect action between an excess of free Pro and growth inhibition in Arabidopsis.

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