HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION (original) (raw)

Journal Article

,

Radiobiology Laboratories

, Yale University School of Medicine, New Haven, Connecticut 06510

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Radiobiology Laboratories

, Yale University School of Medicine, New Haven, Connecticut 06510

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Accepted:

24 December 1985

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ABSTRACT

Using a direct enrichment and screening procedure, mutants of Escherichia coli have been isolated in which recombination frequencies for several intragenic Hfr × F- crosses are significantly higher (twofold to sixfold) than in the parental strains. These hyper-recombination mutations comprised five new mutS - and one new mutL - allele. Together with other known mut - alleles, they were analyzed for effects on intragenic recombination using several types of crosses. Hyper-recombination was found for mutS -, mutL -, mutH (=mutR)- and mutU (=uvrD)-, with the largest effects seen for certain alleles of uvrD; these resulted in over 20-fold excesses in recombinant production for Hfr × F- crosses and F'-chromosome homogenotization. Spontaneous mutator ability was not always correlated with degree of hyper-recombination.

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© Genetics 1986

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