MOLECULAR POPULATION GENETICS OF THE ALCOHOL DEHYDROGENASE GENE REGION OF DROSOPHILA MELANOGASTER (original) (raw)

Journal Article

,

Laboratory of Genetics

, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

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,

Laboratory of Genetics

, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Search for other works by this author on:

,

Laboratory of Genetics

, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, and Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695

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,

Laboratory of Genetics

, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

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Department of Genetics

, North Carolina State University, Raleigh, North Carolina 27695

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Received:

12 January 1985

Accepted:

04 September 1986

Published:

01 December 1986

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Charles F Aquadro, Susan F Desse, Molly M Bland, Charles H Langley, Cathy C Laurie-Ahlberg, MOLECULAR POPULATION GENETICS OF THE ALCOHOL DEHYDROGENASE GENE REGION OF DROSOPHILA MELANOGASTER, Genetics, Volume 114, Issue 4, 1 December 1986, Pages 1165–1190, https://doi.org/10.1093/genetics/114.4.1165
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ABSTRACT

Variation in the DNA restriction map of a 13-kb region of chromosome ll including the alcohol dehydrogenase structural gene (Adh) was examined in Drosophila melanogaster from natural populations. Detailed analysis of 48 D. melanogaster lines representing four eastern United States populations revealed extensive DNA sequence variation due to base substitutions, insertions and deletions. Cloning of this region from several lines allowed characterization of length variation as due to unique sequence insertions or deletions [nine sizes; 21-200 base pairs (bp)] or transposable element insertions (several sizes, 340 bp to 10.2 kb, representing four different elements). Despite this extensive variation in sequences flanking the Adh gene, only one length polymorphism is clearly associated with altered Adh expression (a copia element approximately 250 bp 5′ to the distal transcript start site). Nonetheless, the frequency spectra of transposable elements within and between Drosophila species suggests they are slightly deleterious. Strong nonrandom associations are observed among Adh region sequence variants, ADH allozyme (Fast vs. Slow), ADH enzyme activity and the chromosome inversion ln(2L)t. Phylogenetic analysis of restriction map haplotypes suggest that the major twofold component of ADH activity variation (high vs. low, typical of Fast and Slow allozymes, respectively) is due to sequence variation tightly linked to and possibly distinct from that underlying the allozyme difference. The patterns of nucleotide and haplotype variation for Fast and Slow allozyme lines are consistent with the recent increase in frequency and spread of the Fast haplotype associated with high ADH activity. These data emphasize the important role of evolutionary history and strong nonrandom associations among tightly linked sequence variation as determinants of the patterns of variation observed in natural populations.

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© Genetics 1986

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