Dihydrouridine-deficient tRNAs in Saccharomyces cerevisiae (original) (raw)

Journal Article

,

*

Department of Genetics, University of Alberta

Edmonton, Alberta, Canada T6G 2E9

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,

*

Department of Genetics, University of Alberta

Edmonton, Alberta, Canada T6G 2E9

Search for other works by this author on:

†

Department of Microbiology, University of Alberta

Edmonton, Alberta, Canada T6G 2E9

Search for other works by this author on:

Received:

04 November 1981

Revision received:

22 December 1981

Accepted:

22 December 1981

Published:

11 February 1982

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Abstract

A mutation in Saccharomyces cerevisiae, designated mia, is responsible for the production of isoaccepting tRNA molecules with reduced extents of nucleoside modifications. The mia isoacceptors of tRNAPhe and one of the mutant isoacceptors of tRNATyr were highly purified for nucleoside composition analyses. The data indicate that the mutant isoacceptors are lacking some of the dihydrouridine moieties. This is consistent with our previous hypothesis that the mutant isoacceptors were accumulated due to a defect in a modification process [Lo, R.Y.C. and Bell, J.B. (1981) Current Genetics 3, 73–82]. Data from in vitro poly-U translation experiments also support the previous results, suggesting in vivo biological activity of these mutant tRNAs.

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