S1 sensitive sites in adenovirus DNA (original) (raw)
Journal Article
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Division of Virology, National Institute for Medical Research, Mill Hill
London NW7 1AA, UK
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Division of Virology, National Institute for Medical Research, Mill Hill
London NW7 1AA, UK
Search for other works by this author on:
*Present address: Laboratoire de Génétique Moleculaire des Eucaryotes, Faculté de Médicine, 11 Rue Humann, Strasbourg Cedex, 67085, France
Received:
04 November 1982
Revision received:
09 December 1982
Accepted:
09 December 1982
Published:
11 January 1983
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Abstract
S1 nuclease has been used as a probe for regions of DNA secondary structure in supercoiled recombinant plasmids containing adenovirus (Ad) DNA sequences. In the sequences examined two S1 sensitive sites were identified in the left-terminal 16.5% of Ad 12 DNA, one of which aligned approximately with an inverted repeat region in the DNA sequence. In addition an S1 sensitive site was dictated by a potential cruciform structure in the region of the Ad 2 major late promoter. In contrast to the expected cleavage site at the loop of the cruciform, cleavage occurred at the base of the stem in the region of the TATA box. All three S1 sensitive sites Identified were more sensitive to S1 than the endogenous sites in the parent plasmids.
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Author notes
*Present address: Laboratoire de Génétique Moleculaire des Eucaryotes, Faculté de Médicine, 11 Rue Humann, Strasbourg Cedex, 67085, France
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