Comprehensive genotypic analysis of leukemia: clinical and... : Current Opinion in Oncology (original) (raw)
Leukemia
Comprehensive genotypic analysis of leukemia: clinical and therapeutic implications
Howard Hughes Medical Institute, Brigham and Women's Hospital, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA.
Correspondence to D. Gary Gilliland, Harvard Institutes of Medicine, 4 Blackfan Circle, Room 418, Boston, MA 02115, USA; e-mail: [email protected]
Abstract
Over the past several years, the application of a spectrum of cytogenetic and molecular diagnostic techniques has dramatically improved our understanding of the pathophysiology of leukemia. These techniques include chromosomal translocations visualized by G-banding techniques, fluorescence in-situ hybridization, spectral karyotyping, comparative genomic hybridization, loss of heterozygosity analysis, and characterization of point mutations by DNA sequence analysis. We will review the application of these techniques, update novel findings utilizing these techniques over the past year as they apply to specific leukemias, and review the clinical and therapeutic implications of these findings.
© 2002 Lippincott Williams & Wilkins, Inc.