Histopathologic Correlation of High-Resolution Magnetic... : Investigative Radiology (original) (raw)

Original Investigations

Histopathologic Correlation of High-Resolution Magnetic Resonance Imaging of Human Cervical Tissue Samples at 3 Tesla

Validation of a Technique

CSAPÓ, BENCE MD*∥; SZÉLES, JÓZSEF MD†; HELBICH, THOMAS H. MD‡; KLARHÖFER, MARKUS PhD*; BALÁSSY, CSILLA MD‡; PAMMER, JOHANNES MD§; OBERMAIR, ANDREAS MD¶

From *the NMR-group, Department of Medical Physics, the

†Department of Vascular Surgery, the

‡Department of Radiology, the

§Department of Pathology, and the

¶Department of Gynecology and Obstetrics, University of Vienna, Austria.

∥From the Clinic of Obstetrics and Gynecology, University of Debrecen, Hungary

This study was supported by grant from the Austrian Science Foundation (FWF grant P12041-Med).

Reprint requests to: Thomas H. Helbich, MD, Department of Radiology, University of Vienna, Waehringer Guertel 18-20. 1090-Vienna, Austria; E-mail: [email protected]

Received August 17, 2001, and accepted for publication, after revision, January 23, 2002.

Abstract

Csapó B, Széles J, Helbich TH, et al. Histopathologic correlation of high resolution magnetic resonance imaging of human cervical tissue samples at 3 tesla: validation of a technique. Invest Radiol 2002;37:381–385.

rationale and objectives.

High-resolution magnetic resonance (HR-MR) images of human cervical tissue samples were correlated with low magnification histology to investigate the MR presentation of normal and pathologic anatomic structures in cervical tissue samples at 3T.

methods.

Tissue samples were obtained from five large loop excision of the transformation zone (LLETZ) cone biopsies and one surgical specimen. HR-MR images (3D-Spin Echo sequence: TR/TE: 1500/60 milliseconds, voxel size of 117 × 208 × 234 μm) and low magnification (5×) histology sections were acquired in the same anatomical planes and correlated.

results.

In vitro HR-MR imaging of cervical tissue samples correlated well with low magnification microscopic histology, demonstrating normal anatomy (epithelium and its mucus layer, connective tissue of the stroma, glands, blood vessels). In vitro HR-MR imaging adds information about tumor content and margins.

conclusions.

High resolution MR imaging of cervical tissue samples assists in the identification of both normal structures and pathologic changes.