Male Donor-derived Cells in the Brains of Female Sex-mismatched Bone Marrow Transplant Recipients: A Y-Chromosome Specific In situ Hybridization Study (original) (raw)

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Department of Pathology, Emory University, Atlanta, Georgia,

University of Minnesota

, Minneapolis, Minnesota

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Department of Laboratory Medicine and Pathology, and Neurology,

University of Minnesota

, Minneapolis, Minnesota

Correspondence to: Joo Ho Sung, M.D., Box 157, University of Minnesota Hospital and Clinics, Harvard at East River Road, Minneapolis, MN 55455.

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Department of Laboratory Medicine and Pathology, and Neurology,

University of Minnesota

, Minneapolis, Minnesota

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Department of Pathology, Emory University, Atlanta, Georgia,

University of Minnesota

, Minneapolis, Minnesota

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Department of Pediatrics, Bone Marrow Transplantation, and the Institute of Human Genetics,

University of Minnesota

, Minneapolis, Minnesota

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Department of Pediatrics, Bone Marrow Transplantation, and the Institute of Human Genetics,

University of Minnesota

, Minneapolis, Minnesota

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Published:

01 September 1993

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E. R. Unger, J. H. Sung, J. C. Manivel, M. L. Chenggis, B. R. Blazar, W. Krivit, Male Donor-derived Cells in the Brains of Female Sex-mismatched Bone Marrow Transplant Recipients: A Y-Chromosome Specific In situ Hybridization Study, Journal of Neuropathology & Experimental Neurology, Volume 52, Issue 5, September 1993, Pages 460–470, https://doi.org/10.1097/00005072-199309000-00004
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Abstract

In five female bone marrow transplant (BMT) recipients of sex-mismatched donor marrow, Y-chromosome specific in situ hybridization was performed on formalin-fixed, paraffin-embedded sections of the medulla to detect the male donor marrow-derived cells. Y-chromosome-bearing cells (Y-cells), thereby donor-derived, were matched with leukocyte common antigen (LCA)-reactive cells in adjacent sections immunostained with anti-LCA antibody. Y-cells included mononuclear leukocytes (MNL) within the vessel lumen and infiltrating the perivascular space and parenchyma, and “perivascular cells.” We have, therefore, concluded that donor marrow-derived MNL, though limited in number, do enter the normal-appearing brain and can transform to “perivascular cells” in human BMT recipients. It remains, however, to be confirmed whether MNL entering the normal adult CNS parenchyma transform to ramified microglia.

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© 1993, by the American Association of Neuropathologists, Inc.

Topic:

• in situ hybridization
• bone marrow transplantation
• adult
• cd45 antigens
• chromosomes
• leukocytes, mononuclear
• microglia
• paraffin
• y chromosomes
• antibodies
• brain
• formaldehyde
• parenchyma
• mismatch
• donors

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